ArticleLab on a chip2010
Quantitative analysis of protein translocations by microfluidic total internal reflection fluorescence flow cytometry.
Article in Lab on a chip, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed, 22 citations in OpenAlex.
- Review
- Highly efficient genome editing of human hematopoietic stem cells via a nano-silicon-blade delivery approach.Integrative biology : quantitative biosciences from nano to macro · 2017Article
- Standing surface acoustic wave (SSAW)-based microfluidic cytometer.Lab on a chip · 2014Article
- Optofluidic detection for cellular phenotyping.Lab on a chip · 2012Review
- Live cell imaging of paxillin in rolling neutrophils by dual-color quantitative dynamic footprinting.Microcirculation (New York, N.Y. : 1994) · 2011Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Protein translocation, or the change in a protein's location between different subcellular compartments, is a critical process by which intracellular proteins carry out their cellular functions. Aberrant translocation events contribute to various diseases ranging from metabolic disorders to cancer. In this study, we demonstrate the use of a newly developed single-cell tool, microfluidic total internal reflection fluorescence flow cytometry (TIRF-FC), for detecting both cytosol to plasma membrane and cytosol to nucleus translocations using the tyrosine kinase Syk and the transcription factor NF-κB as models. This technique detects fluorescent molecules at the plasma membrane and in the membrane-proximal cytosol in single cells. We were able to record quantitatively changes in the fluorescence density in the evanescent field associated with these translocation processes for large cell populations with single cell resolution. We envision that TIRF-FC will provide a new approach to explore the molecular biology and clinical relevance of protein translocations.
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Registered trials
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