ArticleIntegrative biology : quantitative biosciences from nano to macro2010
Multiplexed, high-throughput analysis of 3D microtissue suspensions.
Article in Integrative biology : quantitative biosciences from nano to macro, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- Noninvasive andThe Analyst · 2025Article
- Advances in high throughput cell culture technologies for therapeutic screening and biological discovery applications.Bioengineering & translational medicine · 2024Review
- Engineering liver microtissues for disease modeling and regenerative medicine.Advanced functional materials · 2020Article
- Organotypic Modeling of the Tumor Landscape.Frontiers in cell and developmental biology · 2020Review
- Chemoprotection Across the Tumor Border: Cancer Cell Response to Doxorubicin Depends on Stromal Fibroblast Ratios and Interstitial Therapeutic Transport.Cellular and molecular bioengineering · 2017Article
- The Role of Microfluidics for Organ on Chip Simulations.Bioengineering (Basel, Switzerland) · 2017Review
- 3 dimensional cell cultures: a comparison between manually and automatically produced alginate beads.Cytotechnology · 2016Article
- Engineering a perfusable 3D human liver platform from iPS cells.Lab on a chip · 2016Article
- Biomimetic 3D Tissue Models for Advanced High-Throughput Drug Screening.Journal of laboratory automation · 2015Review
- Microfluidic organs-on-chips.Nature biotechnology · 2014Article
- Rapid assessment of migration and proliferation: a novel 3D high-throughput platform for rational and combinatorial screening of tissue-specific biomaterials.Tissue engineering. Part C, Methods · 2014Article
- Micropatterned cell-cell interactions enable functional encapsulation of primary hepatocytes in hydrogel microtissues.Tissue engineering. Part A · 2014Article
- Tunable and dynamic soft materials for three-dimensional cell culture.Soft matter · 2013Article
- Flow-based pipeline for systematic modulation and analysis of 3D tumor microenvironments.Lab on a chip · 2013Article
- Revisiting lab-on-a-chip technology for drug discovery.Nature reviews. Drug discovery · 2012Review
- Microfluidic sorting of microtissues.Biomicrofluidics · 2012Article
- Endogenous fluorescence signatures in living pluripotent stem cells change with loss of potency.PloS one · 2012Article
- Ensemble analysis of angiogenic growth in three-dimensional microfluidic cell cultures.PloS one · 2012Article
- DNA-templated assembly of droplet-derived PEG microtissues.Lab on a chip · 2011Article
- Humanized mice with ectopic artificial liver tissues.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Three-dimensional (3D) tissue models have significantly improved our understanding of structure/function relationships and promise to lead to new advances in regenerative medicine. However, despite the expanding diversity of 3D tissue fabrication methods, approaches for functional assessment have been relatively limited. Here, we describe the fabrication of microtissue (μ-tissue) suspensions and their quantitative evaluation with techniques capable of analyzing large sample numbers and performing multiplexed parallel analysis. We applied this platform to 3D μ-tissues representing multiple stages of liver development and disease including: embryonic stem cells, bipotential hepatic progenitors, mature hepatocytes, and hepatoma cells photoencapsulated in polyethylene glycol hydrogels. Multiparametric μ-tissue cytometry enabled quantitation of fluorescent reporter expression within populations of intact μ-tissues (n≥ 10²-10³) and sorting-based enrichment of subsets for subsequent studies. Further, 3D μ-tissues could be implanted in vivo, respond to systemic stimuli, retrieved and quantitatively assessed. In order to facilitate multiplexed 'pooled' experimentation, fluorescent labeling strategies were developed and utilized to investigate the impact of μ-tissue composition and exposure to soluble factors. In particular, examination of drug/gene interactions on collections of 3D hepatoma μ-tissues indicated synergistic influence of doxorubicin and siRNA knockdown of the anti-apoptotic gene BCL-XL. Collectively, these studies highlight the broad utility of μ-tissue suspensions as an enabling approach for high n, populational analysis of 3D tissue biology in vitro and in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.