ArticleThe Journal of cell biology2010
Dynamic plasticity of large-scale chromatin structure revealed by self-assembly of engineered chromosome regions.
Article in The Journal of cell biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- Review
- Nuclear Compartments: An Incomplete Primer to Nuclear Compartments, Bodies, and Genome Organization Relative to Nuclear Architecture.Cold Spring Harbor perspectives in biology · 2022Review
- Efficient and Reproducible Multigene Expression after Single-Step Transfection Using Improved BAC Transgenesis and Engineering Toolkit.ACS synthetic biology · 2020Article
- Article
- Dynamic interplay between enhancer-promoter topology and gene activity.Nature genetics · 2018Article
- Stable and reproducible transgene expression independent of proliferative or differentiated state using BAC TG-EMBED.Gene therapy · 2018Article
- Bacterial artificial chromosomes establish replication timing and sub-nuclear compartment de novo as extra-chromosomal vectors.Nucleic acids research · 2018Article
- Transcription upregulation via force-induced direct stretching of chromatin.Nature materials · 2016Article
- Cytology of DNA Replication Reveals Dynamic Plasticity of Large-Scale Chromatin Fibers.Current biology : CB · 2016Article
- Two ways to fold the genome during the cell cycle: insights obtained with chromosome conformation capture.Epigenetics & chromatin · 2014Review
- Article
- β-Globin cis-elements determine differential nuclear targeting through epigenetic modifications.The Journal of cell biology · 2013Article
- Measuring transcription dynamics in living cells using fluctuation analysis.Methods in molecular biology (Clifton, N.J.) · 2013Article
- Chromatin-interaction compartment switch at developmentally regulated chromosomal domains reveals an unusual principle of chromatin folding.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Independence of repressive histone marks and chromatin compaction during senescent heterochromatic layer formation.Molecular cell · 2012Article
- Revisiting higher-order and large-scale chromatin organization.Current opinion in cell biology · 2012Review
- Mapping mouse hemangioblast maturation from headfold stages.Developmental biology · 2012Article
- Subnuclear segregation of genes and core promoter factors in myogenesis.Genes & development · 2011Article
- Insights into interphase large-scale chromatin structure from analysis of engineered chromosome regions.Cold Spring Harbor symposia on quantitative biology · 2010Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Interphase chromatin compaction well above the 30-nm fiber is well documented, but the structural motifs underlying this level of chromatin folding remain unknown. Taking a reductionist approach, we analyzed in mouse embryonic stem (ES) cells and ES-derived fibroblasts and erythroblasts the folding of 10-160-megabase pair engineered chromosome regions consisting of tandem repeats of bacterial artificial chromosomes (BACs) containing approximately 200 kilobases of mammalian genomic DNA tagged with lac operator (LacO) arrays. Unexpectedly, linear mitotic and interphase chromatid regions formed from noncontiguously folded DNA topologies. Particularly, in ES cells, these model chromosome regions self-organized with distant sequences segregating into functionally distinct, compact domains. Transcriptionally active and histone H3K27me3-modified regions positioned toward the engineered chromosome subterritory exterior, with LacO repeats and the BAC vector backbone localizing within an H3K9me3, HP1-enriched core. Differential compaction of Dhfr and alpha- and beta-globin transgenes was superimposed on dramatic, lineage-specific reorganization of large-scale chromatin folding, demonstrating a surprising plasticity of large-scale chromatin organization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.