ArticleThe Biochemical journal2010
Interaction with receptor for activated C-kinase 1 (RACK1) sensitizes the phosphodiesterase PDE4D5 towards hydrolysis of cAMP and activation by protein kinase C.
Article in The Biochemical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 35 citations in OpenAlex.
- RACK1 in host immune response to infections: molecular mechanisms and therapeutic potentials.Frontiers in immunology · 2026Review
- PDE4D: A Multipurpose Pharmacological Target.International journal of molecular sciences · 2024Review
- Identification of RACK1 as a novel regulator of non-structural protein 4 of chikungunya virus.Acta biochimica et biophysica Sinica · 2024Article
- RACK1 interaction with c-Src is essential for osteoclast function.Experimental & molecular medicine · 2019Article
- Transcriptomic analysis ofBiology open · 2019Article
- The interaction between RACK1 and WEE1 regulates the growth of gastric cancer cell line HGC27.Oncology letters · 2017Article
- The RNA-binding protein SERBP1 interacts selectively with the signaling protein RACK1.Cellular signalling · 2017Article
- Hypoxia-induced mobilization of NHE6 to the plasma membrane triggers endosome hyperacidification and chemoresistance.Nature communications · 2017Article
- Rack1 Mediates the Interaction of P-Glycoprotein with Anxa2 and Regulates Migration and Invasion of Multidrug-Resistant Breast Cancer Cells.International journal of molecular sciences · 2016Article
- Control of βAR- and N-methyl-D-aspartate (NMDA) Receptor-Dependent cAMP Dynamics in Hippocampal Neurons.PLoS computational biology · 2016Article
- Activation of the cAMP Pathway Induces RACK1-Dependent Binding of β-Actin to BDNF Promoter.PloS one · 2016Article
- The scaffold protein RACK1 mediates the RANKL-dependent activation of p38 MAPK in osteoclast precursors.Science signaling · 2015Article
- RACK1, a versatile hub in cancer.Oncogene · 2015Review
- AChE and RACK1 promote the anti-inflammatory properties of fluoxetine.Journal of molecular neuroscience : MN · 2014Article
- Maize ZmRACK1 is involved in the plant response to fungal phytopathogens.International journal of molecular sciences · 2014Article
- RACK1 is involved in endothelial barrier regulation via its two novel interacting partners.Cell communication and signaling : CCS · 2013Article
- RACK1/Asc1p, a ribosomal node in cellular signaling.Molecular & cellular proteomics : MCP · 2013Article
- Novel cAMP signalling paradigms: therapeutic implications for airway disease.British journal of pharmacology · 2012Review
- RACK1, A multifaceted scaffolding protein: Structure and function.Cell communication and signaling : CCS · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We have previously identified the PKC (protein kinase C)-anchoring protein RACK1 (receptor for activated C-kinase 1), as a specific binding partner for the cAMP-specific phosphodiesterase PDE4D5, suggesting a potential site for cross-talk between the PKC and cAMP signalling pathways. In the present study we found that elevation of intracellular cAMP, with the β₂-adrenoceptor agonist isoproterenol (isoprenaline), led to activation of PDE4 enzymes in the particulate and soluble fractions of HEK (human embryonic kidney)-293 cells. In contrast activation of PDE4D5, with isoproterenol and the PKC activator PMA, was restricted to the particulate fraction, where it interacts with RACK1; however, RACK1 is dispensable for anchoring PDE4D5 to the particulate fraction. Kinetic studies demonstrated that RACK1 alters the conformation of particulate-associated PDE4D5 so that it more readily interacts with its substrate cAMP and with rolipram, a PDE4 inhibitor that specifically targets the active site of the enzyme. Interaction with RACK1 was also essential for PKC-dependent and ERK (extracellular-signal-regulated kinase)-independent phosphorylation (on Ser¹²⁶), and activation of PDE4D5 in response to PMA and isoproterenol, both of which trigger the recruitment of PKCα to RACK1. Together these results reveal novel signalling cross-talk, whereby RACK1 mediates PKC-dependent activation of PDE4D5 in the particulate fraction of HEK-293 cells in response to elevations in intracellular cAMP.
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Registered trials
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