ArticleThe EMBO journal2010
An in vivo RNAi assay identifies major genetic and cellular requirements for primary piRNA biogenesis in Drosophila.
Article in The EMBO journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 166 papers.
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Who cites it
166 citing papers in PubMed, 271 citations in OpenAlex.
- Host-transposable element coexistence: a matter of resistance, tolerance and trade-off.The EMBO journal · 2026Review
- PIWI-Interacting RNAs in brain health and disease: biogenesis, mechanisms, and therapeutic horizons.Psychopharmacology · 2026Review
- MiSiPi.Rna: an integrated tool for characterizing small regulatory RNA processing.RNA (New York, N.Y.) · 2026Article
- A second genetic screen for gurken mRNA mislocalisation uncovers novel phenotypes of piRNA pathway mutants in Drosophila.Biology open · 2025Article
- Molecular insights into the Drosophila piRNA pathway via systematic ReLo protein interaction screening and structure prediction.Nucleic acids research · 2025Article
- piRNA processing within non-membrane structures is governed by constituent proteins and their functional motifs.The FEBS journal · 2025Review
- Evolution of piRNA-guided defense against transposable elements.Trends in genetics : TIG · 2025Review
- Polycomb-mediated transgenerational epigenetic inheritance ofOpen biology · 2025Article
- The Dystrophin-Dystroglycan complex ensures cytokinesis efficiency in Drosophila epithelia.EMBO reports · 2025Article
- piRNA Defense Against Endogenous Retroviruses.Viruses · 2024Review
- TransgenicPharmaceuticals (Basel, Switzerland) · 2024Article
- What goes up must come down: off switches for regulatory RNAs.Genes & development · 2024Review
- Fas2EB112: a tale of two chromosomes.G3 (Bethesda, Md.) · 2024Article
- piRNA generation is associated with the pioneer round of translation in stem cells.Nucleic acids research · 2024Article
- Article
- piRNA-18 Inhibition Cell Proliferation, Migration and Invasion in Colorectal Cancer.Biochemical genetics · 2023Article
- Themes and variations on piRNA-guided transposon control.Mobile DNA · 2023Review
- Evolutionary history of MEK1 illuminates the nature of deleterious mutations.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- The absence of core piRNA biogenesis factors does not impact efficient transposon silencing in Drosophila.PLoS biology · 2023Article
- The emerging role of the piRNA/PIWI complex in respiratory tract diseases.Respiratory research · 2023Review
106 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Drosophila, PIWI proteins and bound PIWI-interacting RNAs (piRNAs) form the core of a small RNA-mediated defense system against selfish genetic elements. Within germline cells, piRNAs are processed from piRNA clusters and transposons to be loaded into Piwi/Aubergine/AGO3 and a subset of piRNAs undergoes target-dependent amplification. In contrast, gonadal somatic support cells express only Piwi, lack signs of piRNA amplification and exhibit primary piRNA biogenesis from piRNA clusters. Neither piRNA processing/loading nor Piwi-mediated target silencing is understood at the genetic, cellular or molecular level. We developed an in vivo RNAi assay for the somatic piRNA pathway and identified the RNA helicase Armitage, the Tudor domain containing RNA helicase Yb and the putative nuclease Zucchini as essential factors for primary piRNA biogenesis. Lack of any of these proteins leads to transposon de-silencing, to a collapse in piRNA levels and to a failure in Piwi-nuclear accumulation. We show that Armitage and Yb interact physically and co-localize in cytoplasmic Yb bodies, which flank P bodies. Loss of Zucchini leads to an accumulation of Piwi and Armitage in Yb bodies, indicating that Yb bodies are sites of primary piRNA biogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.