ArticleApplied and environmental microbiology2010
Towards enhanced galactose utilization by Lactococcus lactis.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 72 citations in OpenAlex.
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- Effect of Enzymatic Lactose Hydrolysis on the Quality and Texture of Full-Fat Curd Cheese Produced Without Whey Separation.Microorganisms · 2025Article
- The Role of Whey in Functional Microorganism Growth and Metabolite Generation: A Biotechnological Perspective.Foods (Basel, Switzerland) · 2025Review
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- Development of SacB-based counterselection for efficient allelic exchange inMicrobiology spectrum · 2025Article
- Environmental and genetic regulation of Streptococcus pneumoniae galactose catabolic pathways.Nature communications · 2024Article
- A New Method for Gene Deletion to Investigate Cell Wall Biogenesis in Fusobacterium nucleatum.Methods in molecular biology (Clifton, N.J.) · 2024Article
- HicA Toxin-Based Counterselection Marker for Allelic Exchange Mutations in Fusobacterium nucleatum.Applied and environmental microbiology · 2023Article
- Transcriptomic profiling reveals differences in the adaptation of two Tetragenococcus halophilus strains to a lupine moromi model medium.BMC microbiology · 2023Article
- Effect of sugar transporter on galactose utilization inFrontiers in microbiology · 2023Article
- Trade-offs predicted by metabolic network structure give rise to evolutionary specialization and phenotypic diversification.Molecular biology and evolution · 2022Article
- Inactivation of GalU Leads to a Cell Wall-Associated Polysaccharide Defect That Reduces the Susceptibility of Enterococcus faecalis to Bacteriolytic Agents.Applied and environmental microbiology · 2021Article
- Role of the pyruvate metabolic network on carbohydrate metabolism and virulence in Streptococcus pneumoniae.Molecular microbiology · 2020Article
- Impact of wilting and additives on fermentation quality and carbohydrate composition of mulberry silage.Asian-Australasian journal of animal sciences · 2020Article
- A Novel Method for Long-Term Analysis of Lactic Acid and Ammonium Production in Non-growingFrontiers in bioengineering and biotechnology · 2020Article
- Presence of galactose in precultures induces lacS and leads to short lag phase in lactose-grown Lactococcus lactis cultures.Journal of industrial microbiology & biotechnology · 2019Article
- Further Elucidation of Galactose Utilization inFrontiers in microbiology · 2018Article
- Expanding the Genetic Code ofFrontiers in microbiology · 2018Article
- TheFrontiers in microbiology · 2017Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Accumulation of galactose in dairy products due to partial lactose fermentation by lactic acid bacteria yields poor-quality products and precludes their consumption by individuals suffering from galactosemia. This study aimed at extending our knowledge of galactose metabolism in Lactococcus lactis, with the final goal of tailoring strains for enhanced galactose consumption. We used directed genetically engineered strains to examine galactose utilization in strain NZ9000 via the chromosomal Leloir pathway (gal genes) or the plasmid-encoded tagatose 6-phosphate (Tag6P) pathway (lac genes). Galactokinase (GalK), but not galactose permease (GalP), is essential for growth on galactose. This finding led to the discovery of an alternative route, comprising a galactose phosphotransferase system (PTS) and a phosphatase, for galactose dissimilation in NZ9000. Introduction of the Tag6P pathway in a galPMK mutant restored the ability to metabolize galactose but did not sustain growth on this sugar. The latter strain was used to prove that lacFE, encoding the lactose PTS, is necessary for galactose metabolism, thus implicating this transporter in galactose uptake. Both PTS transporters have a low affinity for galactose, while GalP displays a high affinity for the sugar. Furthermore, the GalP/Leloir route supported the highest galactose consumption rate. To further increase this rate, we overexpressed galPMKT, but this led to a substantial accumulation of α-galactose 1-phosphate and α-glucose 1-phosphate, pointing to a bottleneck at the level of α-phosphoglucomutase. Overexpression of a gene encoding α-phosphoglucomutase alone or in combination with gal genes yielded strains with galactose consumption rates enhanced up to 50% relative to that of NZ9000. Approaches to further improve galactose metabolism are discussed.
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