ArticleJournal of bacteriology2010
Regulation of the biosynthesis of the macrolide antibiotic spiramycin in Streptomyces ambofaciens.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
- The PucR-type activator IppR regulates 2-isopropylphenol hydroxylation in strainApplied and environmental microbiology · 2026Article
- Enhancement of carrimycin production in Streptomyces spiramyceticus 54-IA by the reporter-guided selection method with traditional mutagenesis.BMC microbiology · 2025Article
- Interplay between Non-Coding RNA Transcription, Stringent/Relaxed Phenotype and Antibiotic Production inAntibiotics (Basel, Switzerland) · 2021Article
- Endophytic actinobacteria of a halophytic desert plant3 Biotech · 2020Article
- Regulation of γ-Aminobutyrate (GABA) Utilization inFrontiers in microbiology · 2020Article
- Engineering actinomycetes for biosynthesis of macrolactone polyketides.Microbial cell factories · 2019Review
- Engineering of leucine-responsive regulatory protein improves spiramycin and bitespiramycin biosynthesis.Microbial cell factories · 2019Article
- Stimulatory Effects of Methyl-β-cyclodextrin on Spiramycin Production and Physical-Chemical Characterization of Nonhost@Guest Complexes.ACS omega · 2018Article
- Influence of dextrins on the production of spiramycin and impurity components by Streptomyces ambofaciens.Folia microbiologica · 2018Article
- Genetic manipulation of secondary metabolite biosynthesis for improved production in Streptomyces and other actinomycetes.Journal of industrial microbiology & biotechnology · 2016Review
- Manipulating natural product biosynthetic pathways via DNA assembler.Current protocols in chemical biology · 2014Article
- Genome mining of Streptomyces ambofaciens.Journal of industrial microbiology & biotechnology · 2014Review
- Post-PKS tailoring steps of the spiramycin macrolactone ring in Streptomyces ambofaciens.Antimicrobial agents and chemotherapy · 2013Article
- Molecular regulation of antibiotic biosynthesis in streptomyces.Microbiology and molecular biology reviews : MMBR · 2013Review
- Construction and engineering of large biochemical pathways via DNA assembler.Methods in molecular biology (Clifton, N.J.) · 2013Article
- DNA assembler: a synthetic biology tool for characterizing and engineering natural product gene clusters.Methods in enzymology · 2012Article
- Rapid characterization and engineering of natural product biosynthetic pathways via DNA assembler.Molecular bioSystems · 2011Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Streptomyces ambofaciens synthesizes the macrolide antibiotic spiramycin. The biosynthetic gene cluster for spiramycin has been characterized for S. ambofaciens. In addition to the regulatory gene srmR (srm22), previously identified (M. Geistlich et al., Mol. Microbiol. 6:2019-2029, 1992), three putative regulatory genes had been identified by sequence analysis. Gene expression analysis and gene inactivation experiments showed that only one of these three genes, srm40, plays a major role in the regulation of spiramycin biosynthesis. The disruption of srm22 or srm40 eliminated spiramycin production while their overexpression increased spiramycin production. Expression analysis was performed by reverse transcription-PCR (RT-PCR) for all the genes of the cluster in the wild-type strain and in the srm22 (srmR) and srm40 deletion mutants. The results from the expression analysis, together with the ones from the complementation experiments, indicated that Srm22 is required for srm40 expression, Srm40 being a pathway-specific activator that controls most, if not all, of the spiramycin biosynthetic genes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.