ArticleThe Journal of biological chemistry2010
Secretion stimulates intramembrane proteolysis of a secretory granule membrane enzyme.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- Peptidylglycine α-amidating monooxygenase as a therapeutic target or biomarker for human diseases.British journal of pharmacology · 2022Review
- Novel insights into peptide amidation and amidating activity in the human circulation.Scientific reports · 2021Article
- Physiological signaling in the absence of amidated peptides.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Ciliary and cytoskeletal functions of an ancient monooxygenase essential for bioactive amidated peptide synthesis.Cellular and molecular life sciences : CMLS · 2019Review
- 60 YEARS OF POMC: From POMC and α-MSH to PAM, molecular oxygen, copper, and vitamin C.Journal of molecular endocrinology · 2016Review
- O-Glycosylation of a Secretory Granule Membrane Enzyme Is Essential for Its Endocytic Trafficking.The Journal of biological chemistry · 2016Article
- Early eukaryotic origins for cilia-associated bioactive peptide-amidating activity.Journal of cell science · 2016Article
- Generation of a novel monoclonal antibody that recognizes the alpha (α)-amidated isoform of a valine residue.BMC neuroscience · 2015Article
- Adaptor Protein-1 Complex Affects the Endocytic Trafficking and Function of Peptidylglycine α-Amidating Monooxygenase, a Luminal Cuproenzyme.The Journal of biological chemistry · 2015Article
- AP-1A controls secretory granule biogenesis and trafficking of membrane secretory granule proteins.Traffic (Copenhagen, Denmark) · 2014Article
- Function, therapeutic potential and cell biology of BACE proteases: current status and future prospects.Journal of neurochemistry · 2014Review
- A histidine-rich linker region in peptidylglycine α-amidating monooxygenase has the properties of a pH sensor.The Journal of biological chemistry · 2014Article
- The many substrates of presenilin/γ-secretase.Journal of Alzheimer's disease : JAD · 2011Review
- Probing the production of amidated peptides following genetic and dietary copper manipulations.PloS one · 2011Article
- Signaling from the secretory granule to the nucleus.Critical reviews in biochemistry and molecular biologyReview
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Regulated intramembrane proteolysis, a highly conserved process employed by diverse regulatory pathways, can release soluble fragments that directly or indirectly modulate gene expression. In this study we used pharmacological tools to identify peptidylglycine α-amidating monooxygenase (PAM), a type I secretory granule membrane protein, as a γ-secretase substrate. PAM, an essential enzyme, catalyzes the final step in the synthesis of the majority of neuropeptides that control metabolic homeostasis. Mass spectroscopy was most consistent with the presence of multiple closely spaced NH(2) termini, suggesting that cleavage occurred near the middle of the PAM transmembrane domain. The luminal domains of PAM must undergo a series of prohormone convertase or α-secretase-mediated cleavages before the remaining transmembrane domain/cytosolic domain fragment can undergo a γ-secretase-like cleavage. Cleavage by γ-secretase generates a soluble fragment of the cytosolic domain (sf-CD) that is known to localize to the nucleus. Although PAM sf-CD is unstable in AtT-20 corticotroph tumor cells, it is readily detected in primary rat anterior pituitary cells. PAM isoform expression, which is tissue-specific and developmentally regulated, affects the efficiency with which sf-CD is produced. sf-CD levels are also modulated by the phosphorylation status of the cytosolic domain and by the ability of the cytosolic domain to interact with cytosolic proteins. sf-CD is produced by primary rat anterior pituitary cells in response to secretogogue, suggesting that sf-CD acts as a signaling molecule relaying information about secretion from the secretory granule to the nucleus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.