ArticleNucleic acids research2011
Human single-nucleotide polymorphisms alter p53 sequence-specific binding at gene regulatory elements.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 31 citations in OpenAlex.
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- Reasoning over genetic variance information in cause-and-effect models of neurodegenerative diseases.Briefings in bioinformatics · 2016Article
- An African-specific polymorphism in the TP53 gene impairs p53 tumor suppressor function in a mouse model.Genes & development · 2016Article
- Genetic Modifiers of the p53 Pathway.Cold Spring Harbor perspectives in medicine · 2016Review
- Genome-wide analysis of p53 transcriptional programs in B cells upon exposure to genotoxic stress in vivo.Oncotarget · 2015Article
- Interactions of chromatin context, binding site sequence content, and sequence evolution in stress-induced p53 occupancy and transactivation.PLoS genetics · 2015Article
- Linking polymorphic p53 response elements with gene expression in airway epithelial cells of smokers and cancer risk.Human genetics · 2014Article
- Chromatin structure is distinct between coding and non-coding single nucleotide polymorphisms.BMC molecular biology · 2014Article
- A comprehensive and high-resolution genome-wide response of p53 to stress.Cell reports · 2014Article
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- Implication of DNA repair genes in prostate tumourigenesis in Indian males.The Indian journal of medical research · 2012Article
- Computational refinement of functional single nucleotide polymorphisms associated with ATM gene.PloS one · 2012Article
- Targeted isolation of cloned genomic regions by recombineering for haplotype phasing and isogenic targeting.Nucleic acids research · 2011Article
- Transcriptional modulation induced by ionizing radiation: p53 remains a central player.Molecular oncology · 2011Review
- p53 Isoforms: An Intracellular Microprocessor?Genes & cancer · 2011Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
p53 coordinates the expression of an intricate network of genes in response to stress signals. Sequence-specific DNA binding is essential for p53-mediated tumor suppression. We evaluated the impact of single-nucleotide polymorphisms (SNPs) in p53 response elements (p53RE) on DNA binding and gene expression in response to DNA damage. Using a bioinformatics approach based on incorporating p53 binding strength into a position weight matrix, we selected 32 SNPs in putative and validated p53REs. The microsphere assay for protein-DNA binding (MAPD) and allele-specific expression analysis was employed to assess the impact of SNPs on p53-DNA binding and gene expression, respectively. Comparing activated p53 binding in nuclear extracts from doxorubicin- or ionizing radiation (IR)-treated human cells, we observed little difference in binding profiles. Significant p53 binding was observed for most polymorphic REs and several displayed binding comparable to the p21 RE. SNP alleles predicted to lower p53 binding indeed reduced binding in 25 of the 32 sequences. Chromatin immunoprecipitation-sequencing in lymphoblastoid cells confirmed p53 binding to seven polymorphic p53 REs in response to doxorubicin. In addition, five polymorphisms were associated with altered gene expression following doxorubicin treatment. Our findings demonstrate an effective strategy to identify and evaluate SNPs that may alter p53-mediated stress responses.
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