ArticleHistochemistry1990
Immunolocalization of ecto-5'-nucleotidase in the kidney by a monoclonal antibody.
Article in Histochemistry, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 54 citations in OpenAlex.
- Sema3dInternational journal of biological sciences · 2026Article
- Extracellular Nucleotides and P2 Receptors in Renal Function.Physiological reviews · 2020Review
- Chronic hypoxia-inducible transcription factor-2 activation stably transforms juxtaglomerular renin cells into fibroblast-like cells in vivo.Journal of the American Society of Nephrology : JASN · 2015Article
- Purinergic signalling in the kidney in health and disease.Purinergic signalling · 2014Review
- Wnt4/β-catenin signaling in medullary kidney myofibroblasts.Journal of the American Society of Nephrology : JASN · 2013Article
- Role of purinergic signaling pathways in V-ATPase recruitment to apical membrane of acidifying epididymal clear cells.American journal of physiology. Cell physiology · 2010Article
- Ectonucleotidases in the kidney.Purinergic signalling · 2009Article
- Effects of increased renal tubular vascular endothelial growth factor (VEGF) on fibrosis, cyst formation, and glomerular disease.The American journal of pathology · 2009Article
- The renal cortical interstitium: morphological and functional aspects.Histochemistry and cell biology · 2008Review
- Origin of renal myofibroblasts in the model of unilateral ureter obstruction in the rat.Histochemistry and cell biology · 2008Article
- Purinergic signaling in the lumen of a normal nephron and in remodeled PKD encapsulated cysts.Purinergic signalling · 2008Article
- Characterization of renal interstitial fibroblast-specific protein 1/S100A4-positive cells in healthy and inflamed rodent kidneys.Histochemistry and cell biology · 2005Article
- Surface expression, polarization, and functional significance of CD73 in human intestinal epithelia.The Journal of clinical investigation · 1997Article
- Morphology of interstitial cells in the healthy kidney.Anatomy and embryology · 1996Review
- Ecto-5'-nucleotidase is expressed by pericytes and fibroblasts in the rat heart.Histochemistry and cell biology · 1995Article
- Distribution of ecto-5'-nucleotidase in the rat liver: effect of anaemia.Histochemistry · 1994Article
- 5'-nucleotidase in spinal meningeal compartments in the rat. An immuno and enzyme histochemical study.Histochemistry · 1992Article
- 5'-Nucleotidase: molecular structure and functional aspects.The Biochemical journal · 1992Review
- Structure-function correlations in erythropoietin formation and oxygen sensing in the kidney.Klinische Wochenschrift · 1991Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A monoclonal antibody IgG, has been raised against ecto-5'-nucleotidase purified from rat kidney homogenate. The specificity of the antibody was verified by immunoprecipitation. The distribution of the corresponding antigen in the rat kidney was studied by immunocytochemistry (FITC and PAP technique) in 1 micron thick cryostat sections. The antibody reacted with the brush border of proximal tubules, the apical cell membrane and the apical cytoplasm of intercalated cells in connecting tubules and collecting ducts and with interstitial cells of the cortex. Among the interstitial cells exclusively stellate shaped fibroblasts were reactive whereas rounded interstitial cells (type II interstitial cells) as well as pericytes and endothelial cells of peritubular capillaries were unreactive. Compared to the staining intensity of the fibroblasts in the cortical labyrinth the reactivity of the fibroblasts in the medullary rays of the cortex was weak or absent. Interstitial cells of the entire medulla were unreactive. Concerning the fibroblasts in the periarterial connective tissue, those surrounding the larger arteries (arcuate arteries, cortical radial arteries) were negative, those alongside afferent and efferent arterioles were positive. Endothelia of lymphatic capillaries travelling within the periarterial connective tissue were also positive. All components of the juxtaglomerular apparatus were negative. The findings are consistent with an interstitial production of adenosine, available extracellularly and thus being able to reach the major target sites of adenosine, the smooth muscles of glomerular arterioles, including the granular cells at the glomerular vascular pole.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.