Evidence map›Paper›PMID 20816475›Full record

ArticleMethods in enzymology2010

Glycosaminoglycan characterization by electrospray ionization mass spectrometry including fourier transform mass spectrometry.

Tatiana N Laremore, Franklin E Leach, Kemal Solakyildirim, I Jonathan Amster, Robert J Linhardt

Abstract read
In one paragraph

Article in Methods in enzymology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed.

  1. Article
  2. Analysis of the Glycosaminoglycan Chains of Proteoglycans.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2021
    Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Review
  9. Article
  10. Article
  11. Article
  12. Proteoglycan sequence.Molecular bioSystems · 2012
    Review
  13. Sodium hydroxide permethylation of heparin disaccharides.Rapid communications in mass spectrometry : RCM · 2011
    Article
  14. Article
  15. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Tatiana N LaremoreDepartment of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, Troy, New York, USA.
Franklin E Leach
Kemal Solakyildirim
I Jonathan Amster
Robert J Linhardt

Funding

Development of a Bioengineered Heparin from a Non-Animal SourceR01HL096972 · NHLBI · RENSSELAER POLYTECHNIC INSTITUTE · PI DORDICK, JONATHAN S., LINHARDT, ROBERT J · 2009 to 2013
$3.9M
SEQUENCING THE POLYSACCHARIDE COMPONENT OF PROTEOGLYCANSR01GM038060 · NIGMS · UNIVERSITY OF IOWA · PI LINHARDT, ROBERT J · 1988 to 2012
$2.8M
Metabolic Engineering of Chinese Hamster Ovary Cells for Production of HeparinR01GM090127 · NIGMS · STATE UNIVERSITY OF NEW YORK AT ALBANY · PI SHARFSTEIN, SUSAN T · 2009 to 2011
$1.0M
NHLBI NIH HHS HL096972NHLBI NIH HHS R01 HL096972NIGMS NIH HHS GM090127NIGMS NIH HHS GM38060NIGMS NIH HHS R01 GM038060NIGMS NIH HHS R01 GM090127
6 · The paper itself

Abstract

Electrospray ionization mass spectrometry (ESI MS) is a versatile analytical technique in glycomics of glycosaminoglycans (GAGs). Combined with enzymology, ESI MS is used for assessing changes in disaccharide composition of GAGs biosynthesized under different environmental or physiological conditions. ESI coupled with high-resolution mass analyzers such as a Fourier transform mass spectrometer (FTMS) permits accurate mass measurement of large oligosaccharides and intact GAGs as well as structural characterization of GAG oligosaccharides using information-rich fragmentation methods such as electron detachment dissociation. The first part of this chapter describes methods for disaccharide compositional profiling using ESI MS and the second part is dedicated to FTMS and tandem MS methods of GAG compositional and structural analysis.

Indexed as

Spectrometry, Mass, Electrospray IonizationSpectroscopy, Fourier Transform InfraredGlycosaminoglycansHumansGlycosaminoglycans

Identifiers

PMID20816475
PMCPMC3234587

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.