Evidence map›Paper›PMID 20816160›Full record

ArticleMethods in enzymology2010

Transcript analysis of stem cells.

Alison V Nairn, Mitche dela Rosa, Kelley W Moremen

Abstract read
In one paragraph

Article in Methods in enzymology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. The Journal of biological chemistry · 2018
    Article
  8. Article
  9. Article
  10. Article
  11. The Journal of biological chemistry · 2017
    Article
  12. Article
  13. Sialylation regulates brain structure and function.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2015
    Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Vertebrate protein glycosylation: diversity, synthesis and function.Nature reviews. Molecular cell biology · 2012
    Review
  19. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Alison V NairnThe Complex Carbohydrate Research Center and the Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, USA.
Mitche dela Rosa
Kelley W Moremen

Funding

ZINC DEFICIENCY &PROTEIN EXPRESSION IN RAT OLFACTORY BULBSP41RR018502 · NCRR · UNIVERSITY OF GEORGIA (UGA) · PI PIERCE, J. MICHAEL · 2003 to 2011
$13.1M
NCRR NIH HHS P41 RR018502NCRR NIH HHS RR018502
6 · The paper itself

Abstract

Quantitative real-time polymerase chain reaction (qRT-PCR) is a flexible and scalable method for analyzing transcript abundance that can be used at a single gene or high-throughput (>100 genes) level. Information obtained from this technique can be used as an indicator of potential regulation of glycosylation at the transcript level when combined with glycan structural or protein abundance data. This chapter describes detailed methods to design and perform qRT-PCR analyses and provides examples of information that can be obtained from the technique.

Indexed as

Gene Expression RegulationStem CellsAnimalsCell DifferentiationGene Expression ProfilingGenetic MarkersGene Transfer TechniquesHumansMicePolysaccharidesReverse Transcriptase Polymerase Chain ReactionGenetic MarkersPolysaccharides

Identifiers

PMID20816160
PMCPMC3104320

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.