ArticleBiophysical journal2010
Temperature modulation of integrin-mediated cell adhesion.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 70 citations in OpenAlex.
- Isolation of high-purity muscle stem cells through ice-cold treatment method for the production of cell cultured meat.Current research in food science · 2025Article
- Positive and Relaxed Selective Pressures Have Both Strongly Influenced the Evolution of Cryonotothenioid Fishes during Their Radiation in the Freezing Southern Ocean.Genome biology and evolution · 2023Article
- Molecular recognition of a membrane-anchored HIV-1 pan-neutralizing epitope.Communications biology · 2022Article
- May the force be with your (immune) cells: an introduction to traction force microscopy in Immunology.Frontiers in immunology · 2022Review
- Cell mechanical properties of human breast carcinoma cells depend on temperature.Scientific reports · 2021Article
- Controlling T cells spreading, mechanics and activation by micropatterning.Scientific reports · 2021Article
- A novel approach for the isolation and long-term expansion of pure satellite cells based on ice-cold treatment.Skeletal muscle · 2021Article
- Characterization of Human NK Cell-Derived Exosomes: Role of DNAM1 Receptor In Exosome-Mediated Cytotoxicity Against Tumor.Cancers · 2020Article
- Cellogram: On-the-Fly Traction Force Microscopy.Nano letters · 2019Article
- Hyaluronic acid selective anchoring to the cytoskeleton: An atomic force microscopy study.PloS one · 2018Article
- α6 Integrin (α6International journal of molecular sciences · 2017Article
- Reinforcement of integrin-mediated T-Lymphocyte adhesion by TNF-induced Inside-out Signaling.Scientific reports · 2016Article
- Directional bleb formation in spherical cells under temperature gradient.Biophysical journal · 2015Article
- A microfluidic device for label-free, physical capture of circulating tumor cell clusters.Nature methods · 2015Article
- Article
- Myosin II Activity Softens Cells in Suspension.Biophysical journal · 2015Article
- Effect of Storage Temperature on Key Functions of Cultured Retinal Pigment Epithelial Cells.Journal of ophthalmology · 2015Article
- Impact of heating on passive and active biomechanics of suspended cells.Interface focus · 2014Article
- AFM of the ultrastructural and mechanical properties of lipid-raft-disrupted and/or cold-treated endothelial cells.The Journal of membrane biology · 2014Article
- Biomechanics and the thermotolerance of development.PloS one · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
In response to external stimuli, cells modulate their adhesive state by regulating the number and intrinsic affinity of receptor/ligand bonds. A number of studies have shown that cell adhesion is dramatically reduced at room or lower temperatures as compared with physiological temperature. However, the underlying mechanism that modulates adhesion is still unclear. Here, we investigated the adhesion of the monocytic cell line THP-1 to a surface coated with intercellular adhesion molecule-1 (ICAM-1) as a function of temperature. THP-1 cells express the integrin lymphocyte function-associated antigen-1 (LFA-1), a receptor for ICAM-1. Direct force measurements of cell adhesion and cell elasticity were carried out by atomic force microscopy. Force measurements revealed an increase of the work of de-adhesion with temperature that was coupled to a gradual decrease in cellular stiffness. Of interest, single-molecule measurements revealed that the rupture force of the LFA-1/ICAM-1 complex decreased with temperature. A detailed analysis of the force curves indicated that temperature-modulated cell adhesion was mainly due to the enhanced ability of cells to deform and to form a greater number of longer membrane tethers at physiological temperatures. Together, these results emphasize the importance of cell mechanics and membrane-cytoskeleton interaction on the modulation of cell adhesion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.