ArticleBMC research notes2010
Towards the simplification of MHC typing protocols: targeting classical MHC class II genes in a passerine, the pied flycatcher Ficedula hypoleuca.
Article in BMC research notes, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
What it found
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Meta-analysis of major histocompatibility complex (MHC) class IIA reveals polymorphism and positive selection in many vertebrate species.Molecular ecology · 2022Pooled it
- Distinct evolutionary trajectories of MHC class I and class II genes in Old World finches and buntings.Heredity · 2021Article
- Molecular Characterization of MHC Class I Genes in Four Species of theBioMed research international · 2021Article
- Genotyping strategy matters when analyzing hypervariable major histocompatibility complex-Experience from a passerine bird.Ecology and evolution · 2018Article
- MHC class II β exon 2 variation in pardalotes (Pardalotidae) is shaped by selection, recombination and gene conversion.Immunogenetics · 2017Article
- Characterization of class II β chain major histocompatibility complex genes in a family of Hawaiian honeycreepers: 'amakihi (Hemignathus virens).Immunogenetics · 2016Article
- Major histocompatibility complex class II DAB alleles associated with intestinal parasite load in the vulnerable Chinese egret (Egretta eulophotes).Ecology and evolution · 2016Article
- Balancing selection and recombination as evolutionary forces caused population genetic variations in golden pheasant MHC class I genes.BMC evolutionary biology · 2016Article
- Non-neutral evolution and reciprocal monophyly of two expressed Mhc class II B genes in Leach's storm-petrel.Immunogenetics · 2015Article
- Major histocompatibility complex alleles associated with parasite susceptibility in wild giant pandas.Heredity · 2015Article
- Characterization of MHC class II B polymorphism in bottlenecked New Zealand saddlebacks reveals low levels of genetic diversity.Immunogenetics · 2013Article
- MHC class II B diversity in blue tits: a preliminary study.Ecology and evolution · 2013Article
- Article
- Maintenance of MHC Class IIB diversity in a recently established songbird population.Journal of avian biology · 2012Article
- 454 sequencing reveals extreme complexity of the class II Major Histocompatibility Complex in the collared flycatcher.BMC evolutionary biology · 2010Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMajor Histocompatibility Complex (MHC) has drawn the attention of evolutionary biologists due to its importance in crucial biological processes, such as sexual selection and immune response in jawed vertebrates. However, the characterization of classical MHC genes subjected to the effects of natural selection still remains elusive in many vertebrate groups. Here, we have tested the suitability of flanking intron sequences to guide the selective exploration of classical MHC genes driving the co-evolutionary dynamics between pathogens and their passerine (Aves, Order Passeriformes) hosts.
findingsIntronic sequences flanking the usually polymorphic exon 2 were isolated from different species using primers sitting on conserved coding regions of MHC class II genes (β chain). Taking the pied flycatcher Ficedula hypoleuca as an example, we demonstrate that careful primer design can evade non-classical MHC gene and pseudogene amplification. At least four polymorphic and expressed loci were co-replicated using a single pair of primers in five non-related individuals (N = 28 alleles). The cross-amplification and preliminary inspection of similar MHC fragments in eight unrelated songbird taxa suggests that similar approaches can also be applied to other species.
conclusionsIntron sequences flanking the usually polymorphic exon 2 may assist the specific investigation of classical MHC class II B genes in species characterized by extensive gene duplication and pseudogenization. Importantly, the evasion of non-classical MHC genes with a more specific function and non-functional pseudogenes may accelerate data collection and diminish lab costs. Comprehensive knowledge of gene structure, polymorphism and expression profiles may be useful not only for the selective examination of evolutionarily relevant genes but also to restrict chimera formation by minimizing the number of co-amplifying loci.
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