ArticleThe Biochemical journal2010
Functional domains of yeast hexokinase 2.
Article in The Biochemical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 53 citations in OpenAlex.
- Review
- Multilayered control of hexose uptake and phosphorylation through specialized sugar kinases in Nakaseomyces glabratus.Fungal biology and biotechnology · 2026Article
- Ovoid cell is an inducible small-sized morphotype that enhances proliferation and antifungal drug tolerance in the human fungal pathogen Cryptococcus neoformans.PLoS pathogens · 2026Article
- Changing course: Glucose starvation drives nuclear accumulation of Hexokinase 2 in S. cerevisiae.PLoS genetics · 2023Article
- Proteomic consequences of TDA1 deficiency in Saccharomyces cerevisiae: Protein kinase Tda1 is essential for Hxk1 and Hxk2 serine 15 phosphorylation.Scientific reports · 2022Article
- AMPK Phosphorylation Is Controlled by Glucose Transport Rate in a PKA-Independent Manner.International journal of molecular sciences · 2021Article
- Article
- A 37-amino acid loop in the Yarrowia lipolytica hexokinase impacts its activity and affinity and modulates gene expression.Scientific reports · 2021Article
- Auxin-mediated protein depletion for metabolic engineering in terpene-producing yeast.Nature communications · 2021Article
- Spontaneous mutations that confer resistance to 2-deoxyglucose act through Hxk2 and Snf1 pathways to regulate gene expression and HXT endocytosis.PLoS genetics · 2020Article
- Laboratory evolution for forced glucose-xylose co-consumption enables identification of mutations that improve mixed-sugar fermentation by xylose-fermenting Saccharomyces cerevisiae.FEMS yeast research · 2018Article
- Article
- Diacylglycerol triggers Rim101 pathway-dependent necrosis in yeast: a model for lipotoxicity.Cell death and differentiation · 2018Article
- The Ability of a Charophyte Alga Hexokinase to Restore Glucose Signaling and Glucose Repression of Gene Expression in a Glucose-InsensitiveFrontiers in plant science · 2018Article
- Conformational Characteristics of Rice Hexokinase OsHXK7 as a Moonlighting Protein involved in Sugar Signalling and Metabolism.The protein journal · 2017Article
- The Expanding Landscape of Moonlighting Proteins in Yeasts.Microbiology and molecular biology reviews : MMBR · 2016Review
- Hexokinase 2 Is an Intracellular Glucose Sensor of Yeast Cells That Maintains the Structure and Activity of Mig1 Protein Repressor Complex.The Journal of biological chemistry · 2016Article
- Glucose repression in Saccharomyces cerevisiae.FEMS yeast research · 2015Review
- A Minimal Set of Glycolytic Genes Reveals Strong Redundancies in Saccharomyces cerevisiae Central Metabolism.Eukaryotic cell · 2015Article
- Network reconstruction and validation of the Snf1/AMPK pathway in baker's yeast based on a comprehensive literature review.NPJ systems biology and applications · 2015Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hkx2 (hexokinase 2) from Saccharomyces cerevisiae was one of the first metabolic enzymes described as a multifunctional protein. Hxk2 has a double subcellular localization: it functions as a glycolytic enzyme in the cytoplasm and as a regulator of gene transcription of several Mig1-regulated genes in the nucleus. To get more insights into the structure-function relationships of the Hxk2 protein, we followed two different approaches. In the first, we deleted the last eight amino acids of Hxk2 and replaced Ser³⁰⁴ with phenylalanine to generate Hxk2(wca). Analysis of this mutant demonstrated that these domains play an essential role in the catalytic activity of yeast Hxk2, but has no effect on the regulatory function of this protein. In the second, we analysed whether amino acids from Lys⁶ to Met¹⁵ of Hxk2 (Hxk2(wrf)) are essential for the regulatory role of Hxk2 and whether there is an effect on the hexose kinase activity of this protein. In the present paper, we report that the Hxk2(wca) mutant protein interacts with the Mig1 transcriptional repressor and the Snf1 protein kinase in the nucleus at the level of the SUC2-Mig1 repressor complex. We have demonstrated that Hxk2(wca) maintained full regulatory function because the glucose-repression signalling of the wild-type machinery is maintained. We also report that the Hxk2(wrf) mutant allele is incapable of glucose repression signalling because it does not interact with Mig1 at the level of the SUC2-Mig1 repressor complex. The two mutants, Hxk2(wca) and Hxk2(wrf) retain single functions, as a transcriptional factor or as an enzyme with hexose-phosphorylating activity, but have lost the original bifunctionality of Hxk2.
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