ArticleProteomics2010
Characterization of the proteomes associating with three distinct membrane raft sub-types in murine sperm.
Article in Proteomics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 72 citations in OpenAlex.
- Genome-wide association study in Japanese females identifies fifteen novel skin-related trait associations.Scientific reports · 2018Trial
- Prominosomes - a particular class of extracellular vesicles containing prominin-1/CD133?Journal of nanobiotechnology · 2025Review
- Bottom-up approach to deciphering the targets of the ubiquitin-proteasome system in porcine sperm capacitation.Scientific reports · 2024Article
- Emerging roles of prominin-1 (CD133) in the dynamics of plasma membrane architecture and cell signaling pathways in health and disease.Cellular & molecular biology letters · 2024Review
- Association between Yili goose sperm motility and expression profiles of mRNA and miRNA in testis.BMC genomics · 2023Article
- Testis-Specific Isoform of NaInternational journal of molecular sciences · 2022Review
- Membrane-Mediated Regulation of Sperm Fertilization Potential in Poultry.The journal of poultry science · 2022Review
- Role of Plasmalemma Microdomains (Rafts) in Protection of the Plant Cell Under Osmotic Stress.The Journal of membrane biology · 2021Article
- Existence and distribution of Niemann-Pick type 2C (NPC2) in prawn reproductive tract and its putative role as a cholesterol modulator during sperm transit in the vas deferens.Cell and tissue research · 2020Article
- Non-detergent Isolation of Membrane Structures from Beet Plasmalemma and Tonoplast Having Lipid Composition Characteristic of Rafts.The Journal of membrane biology · 2020Article
- Characterization of the functions and proteomes associated with membrane rafts in chicken sperm.PloS one · 2017Article
- Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways.Scientific reports · 2016Article
- Comparison of Membrane Characteristics between Freshly Ejaculated and Cryopreserved Sperm in the Chicken.The journal of poultry science · 2016Article
- The F0F1 ATP Synthase Complex Localizes to Membrane Rafts in Gonadotrope Cells.Molecular endocrinology (Baltimore, Md.) · 2016Article
- Organization of Membrane Rafts in Chicken Sperm.The journal of poultry science · 2016Article
- Commentary: "prom1 function in development, intestinal inflammation, and intestinal tumorigenesis".Frontiers in oncology · 2015Article
- The biological networks in studying cell signal transduction complexity: The examples of sperm capacitation and of endocannabinoid system.Computational and structural biotechnology journal · 2014Review
- Membrane rafts regulate phospholipase B activation in murine sperm.Communicative & integrative biology · 2013Article
- Phospholipase B is activated in response to sterol removal and stimulates acrosome exocytosis in murine sperm.The Journal of biological chemistry · 2013Article
- Disruption of a spermatogenic cell-specific mouse enolase 4 (eno4) gene causes sperm structural defects and male infertility.Biology of reproduction · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Mammalian sperm are transcriptionally and translationally inactive. To meet changing needs in the epididymis and female tract, they rely heavily on post-translational modifications and protein acquisition/degradation. Membrane rafts are sterol and sphingolipid-enriched micro-domains that organize and regulate various pathways. Rafts have significance in sperm by transducing the stimulus of sterol efflux into changes in intracellular signaling that confer fertilization competence. We recently characterized three biochemically distinct sub-types of sperm rafts, and now present profiles for proteins targeting to and associating with these sub-types, along with a fraction largely comprised of "non-raft" domains. Proteomics analysis using a gel-based LC-MS/MS approach identified 190 strictly validated proteins in the raft sub-types. Interestingly, many of these are known to be expressed in the epididymis, where sperm membrane composition matures. To investigate potential roles for rafts in epididymal protein acquisition, we compared the expression and localization of two different sterol-interacting proteins, apolipoprotein-A1 (apoA1) and prominin-1 (prom1) in sperm from different zones. We found that apoA1 was gradually added to the plasma membrane overlying the acrosome, whereas prom1 was not, suggesting different mechanisms for raft protein acquisition. Our results define raft-associating proteins, demonstrate functional similarities and differences among raft sub-types, and provide insights into raft-mediated epididymal protein acquisition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.