ArticleCytoskeleton (Hoboken, N.J.)2010
Coupling between microtubule sliding, plus-end growth and spindle length revealed by kinesin-8 depletion.
Article in Cytoskeleton (Hoboken, N.J.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 40 citations in OpenAlex.
- Mechanism of spindle stability and poleward flux regulating spindle length during the metaphase.iScience · 2025Article
- Kinesin-8-specific loop-2 controls the dual activities of the motor domain according to tubulin protofilament shape.Nature communications · 2022Article
- Article
- Review
- Article
- Article
- Emerging Insights into the Function of Kinesin-8 Proteins in Microtubule Length Regulation.Biomolecules · 2018Review
- Discrete regions of the kinesin-8 Kip3 tail differentially mediate astral microtubule stability and spindle disassembly.Molecular biology of the cell · 2018Article
- Article
- Motor axon navigation relies on Fidgetin-like 1-driven microtubule plus end dynamics.The Journal of cell biology · 2018Article
- The Drosophila orthologue of the INT6 onco-protein regulates mitotic microtubule growth and kinetochore structure.PLoS genetics · 2017Article
- Article
- Kinesin-8 effects on mitotic microtubule dynamics contribute to spindle function in fission yeast.Molecular biology of the cell · 2016Article
- Cytoplasmic Determination of Meiotic Spindle Size Revealed by a Unique Inter-Species Germinal Vesicle Transfer Model.Scientific reports · 2016Article
- Inducible fluorescent speckle microscopy.The Journal of cell biology · 2016Article
- Antenna Mechanism of Length Control of Actin Cables.PLoS computational biology · 2015Article
- Role and regulation of kinesin-8 motors through the cell cycle.Systems and synthetic biology · 2014Article
- The kinesin-8 Kip3 scales anaphase spindle length by suppression of midzone microtubule polymerization.The Journal of cell biology · 2014Article
- Patronin mediates a switch from kinesin-13-dependent poleward flux to anaphase B spindle elongation.The Journal of cell biology · 2013Article
- Aurora B and Kif2A control microtubule length for assembly of a functional central spindle during anaphase.The Journal of cell biology · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Mitotic spindle length control requires coordination between microtubule (MT) dynamics and motor-generated forces. To investigate how MT plus-end polymerization contributes to spindle length in Drosophila embryos, we studied the dynamics of the MT plus-end depolymerase, kinesin-8, and the effects of kinesin-8 inhibition using mutants and antibody microinjection. As expected, kinesin-8 was found to contribute to anaphase A. Furthermore, kinesin-8 depletion caused: (i) excessive polymerization of interpolar (ip) MT plus ends, which "overgrow" to penetrate distal half spindles; (ii) an increase in the poleward ipMT sliding rate that is coupled to MT plus-end polymerization; (iii) premature spindle elongation during metaphase/anaphase A; and (iv) an increase in the anaphase B spindle elongation rate which correlates linearly with the MT sliding rate. This is best explained by a revised "ipMT sliding/minus-end depolymerization" model for spindle length control which incorporates a coupling between ipMT plus end dynamics and the outward ipMT sliding that drives poleward flux and spindle elongation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.