ArticleCytoskeleton (Hoboken, N.J.)2010
Segmentation and tracking of cytoskeletal filaments using open active contours.
Article in Cytoskeleton (Hoboken, N.J.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 101 papers.
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Who cites it
101 citing papers in PubMed, 223 citations in OpenAlex.
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- GraFT: A robust network-based spatiotemporal analysis of filamentous structures.Science advances · 2026Article
- From Pixels to Patterns: A Multidimensional Framework to Decode Cytoskeletal Organization.Computational and structural biotechnology journal · 2026Review
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- The wavelength of the ciliary beat in Chlamydomonas saturates at long ciliary lengths.Biophysical journal · 2025Article
- Protein design of two-component tubular assemblies similar to cytoskeletons.Nature communications · 2025Article
- Human formin FHOD3-mediated actin elongation is required for sarcomere integrity in cardiomyocytes.eLife · 2025Article
- Structural response of microtubule and actin cytoskeletons to direct intracellular load.The Journal of cell biology · 2025Article
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- Structural basis of MICAL autoinhibition.Nature communications · 2024Article
- Deciphering the intracellular forces shaping mitochondrial motion.Scientific reports · 2024Article
- KnotResolver: tracking self-intersecting filaments in microscopy using directed graphs.Bioinformatics (Oxford, England) · 2024Article
- Article
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- Single-molecule long-read methylation profiling reveals regional DNA methylation regulated by Elongator Complex Subunit 2 in Arabidopsis roots experiencing spaceflight.Biology direct · 2024Article
- Computational tools for quantifying actin filament numbers, lengths, and bundling.Biology open · 2024Article
- Stabilization of F-actin bybioRxiv : the preprint server for biology · 2023Article
41 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
We use open active contours to quantify cytoskeletal structures imaged by fluorescence microscopy in two and three dimensions. We developed an interactive software tool for segmentation, tracking, and visualization of individual fibers. Open active contours are parametric curves that deform to minimize the sum of an external energy derived from the image and an internal bending and stretching energy. The external energy generates (i) forces that attract the contour toward the central bright line of a filament in the image, and (ii) forces that stretch the active contour toward the ends of bright ridges. Images of simulated semiflexible polymers with known bending and torsional rigidity are analyzed to validate the method. We apply our methods to quantify the conformations and dynamics of actin in two examples: actin filaments imaged by TIRF microscopy in vitro, and actin cables in fission yeast imaged by spinning disk confocal microscopy.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.