ArticleJournal of industrial microbiology & biotechnology2011
Enhancement of extracellular purine nucleoside accumulation by Bacillus strains through genetic modifications of genes involved in nucleoside export.
Article in Journal of industrial microbiology & biotechnology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 32 citations in OpenAlex.
- Efficient production of guanosine in Escherichia coli by combinatorial metabolic engineering.Microbial cell factories · 2024Article
- Exploration of the link between gut microbiota and purinergic signalling.Purinergic signalling · 2023Review
- In silico-guided metabolic engineering of Bacillus subtilis for efficient biosynthesis of purine nucleosides by blocking the key backflow nodes.Biotechnology for biofuels and bioproducts · 2022Article
- Gut microbiota-derived inosine from dietary barley leaf supplementation attenuates colitis through PPARγ signaling activation.Microbiome · 2021Article
- Impairment of Intestinal Barrier Function Induced by Early WeaningFrontiers in immunology · 2021Article
- Expression and purification of the 5'-nucleotidase YitU from Bacillus species: its enzymatic properties and possible applications in biotechnology.Applied microbiology and biotechnology · 2020Article
- Biocontrol UsingFrontiers in microbiology · 2020Article
- Increased production of inosine and guanosine by means of metabolic engineering of the purine pathway in Ashbya gossypii.Microbial cell factories · 2015Article
- Current development in genetic engineering strategies of Bacillus species.Microbial cell factories · 2014Review
- Analysis of cepA encoding an efflux pump-like protein in Corynebacterium glutamicum.Journal of microbiology (Seoul, Korea) · 2014Article
- Complete genome sequence of Bacillus amyloliquefaciens XH7, which exhibits production of purine nucleosides.Journal of bacteriology · 2011Article
- Reconstruction of Purine Metabolism in Bacillus subtilis to Obtain the Strain Producer of AICAR: A New Drug with a Wide Range of Therapeutic Applications.Acta naturae · 2011Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using a simple method to introduce genetic modifications into the chromosome of naturally nontransformable Bacillus, a set of marker-free inosine-producing and 5-aminoimidazole-4-carboxamide (AICA) ribonucleoside-producing Bacillus amyloliquefaciens strains has been constructed. These strains differ in expression levels of the genes responsible for nucleoside export. Overexpression of B. amyloliquefaciens pbuE and heterologous expression of Escherichia coli nepI, which encode nucleoside efflux transporters, each notably enhanced inosine production by a B. amyloliquefaciens nucleoside-producing strain. pbuE overexpression was found to increase AICA ribonucleoside accumulation, indicating that the substrate specificity of the PbuE pump extends to this nucleoside. These results demonstrate that identifying genes whose products facilitate transport of a desired nucleoside out of cells and enhancing their expression can improve the performance of strains used for industrial production.
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