ArticleGenetics2010
When Cre-mediated recombination in mice does not result in protein loss.
Article in Genetics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 33 citations in OpenAlex.
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- Lats1/2 Are Essential for Developmental Vascular Remodeling and Biomechanical Adaptation to Shear Stress.Arteriosclerosis, thrombosis, and vascular biology · 2025Article
- MyD88 signalling in B cells and antibody responses during Oropouche virus-induced neurological disease in mice.EBioMedicine · 2025Article
- Generation of Oviductal Glycoprotein 1 Cre Mouse Model for the Study of Secretory Epithelial Cells of the Oviduct.Endocrinology · 2024Article
- Col1α2-Cre-mediated recombination occurs in various cell types due to Cre expression in epiblasts.Scientific reports · 2023Article
- Erythroid-specific inactivation of Slc12a6/Kcc3 by EpoR promoter-driven Cre expression reduces K-Cl cotransport activity in mouse erythrocytes.Physiological reports · 2022Article
- Transgenic mouse models of breast cancer.Cancer letters · 2021Article
- Optogenetic Control of Airway Cholinergic NeuronsAmerican journal of respiratory cell and molecular biology · 2020Article
- A critical role of PRDM14 in human primordial germ cell fate revealed by inducible degrons.Nature communications · 2020Article
- Aberrant RNA splicing is the major pathogenic effect in a knock-in mouse model of the dominantly inherited c.1430A>G human RPE65 mutation.Human mutation · 2019Article
- Consequences of Cre-mediated deletion of Ciz1 exon 5 in mice.FEBS letters · 2018Article
- β-catenin is required in the neural crest and mesencephalon for pituitary gland organogenesis.BMC developmental biology · 2016Article
- De novo generation of adipocytes from circulating progenitor cells in mouse and human adipose tissue.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2016Article
- Shp2 Associates with and Enhances Nephrin Tyrosine Phosphorylation and Is Necessary for Foot Process Spreading in Mouse Models of Podocyte Injury.Molecular and cellular biology · 2016Article
- Spontaneous Formation of Extensive Vessel-Like Structures in Murine Engineered Heart Tissue.Tissue engineering. Part A · 2016Article
- Generation of a Retinoblastoma (Rb)1-inducible dominant-negative (DN) mouse model.Frontiers in cellular neuroscience · 2015Article
- Generation of mice with conditional ablation of the Cd40lg gene: new insights on the role of CD40L.Transgenic research · 2014Article
- Heritable and inducible gene knockdown in astrocytes or neurons in vivo by a combined lentiviral and RNAi approach.Frontiers in cellular neuroscience · 2014Article
- Inducible control of gene expression with destabilized Cre.Nature methods · 2013Article
- VEGF and podocytes in diabetic nephropathy.Seminars in nephrology · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
Abstract
Cre/loxP recombination enables cellular specificity and, in the case of inducible systems, temporal control of genomic deletions. Here we used a SM22α tamoxifen-inducible Cre line to inactivate β1 integrin in adult smooth muscle. Interestingly, analysis of two distinct β1 loxP transgenic mice revealed vastly different outcomes after β1 integrin deletion. Lethality occurred 4 weeks postinduction in one Cre/loxP line, while no apparent phenotype was seen in the other line. Genetic analysis revealed appropriate DNA excision in both cases; however, differences were found in the degree of protein loss with absolutely no change in protein levels in the model that lacked a phenotype. Seeking to understand protein persistence despite appropriate recombination, we first validated the flox allele using a constitutive Cre line and demonstrated its ability to mediate effective protein inactivation. We then examined the possibility of heterozygous cell selection, protein turnover, and deletion efficiency with no success for explaining the phenotype. Finally, we documented the presence of the Cre-recombination episomal product, which persisted in tissue samples with no protein loss. The product was only noted in cells with low proliferative capacity. These findings highlight the potential for protein expression from the products of Cre-recombinase excised genes, particularly when deletion occurs in low turnover populations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.