ArticleThe Journal of biological chemistry2010
Proliferating cell nuclear antigen (PCNA) is required for cell cycle-regulated silent chromatin on replicated and nonreplicated genes.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 42 citations in OpenAlex.
- Identification and therapeutic efficacy of Pleurotus djamor var fuscopruinosus.Scientific reports · 2025Article
- Additions toMycoKeys · 2023Article
- The Use of Yeast in Biosensing.Microorganisms · 2022Review
- CAF-1 and Rtt101p function within the replication-coupled chromatin assembly network to promote H4 K16ac, preventing ectopic silencing.PLoS genetics · 2020Article
- Re-enforcing hypoxia-induced polyploid cardiomyocytes enter cytokinesis through activation of β-catenin.Scientific reports · 2019Article
- FRET Microscopy in Yeast.Biosensors · 2019Review
- Mutations in the PCNA DNA Polymerase Clamp ofGenetics · 2019Article
- Modulation of Gene Silencing by Cdc7p via H4 K16 Acetylation and Phosphorylation of Chromatin Assembly Factor CAF-1 inGenetics · 2019Article
- Proliferating cell nuclear antigen (PCNA) contributes to the high-order structure and stability of heterochromatin in Saccharomyces cerevisiae.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2017Article
- Mechanisms Underlying Acrolein-Mediated Inhibition of Chromatin Assembly.Molecular and cellular biology · 2016Article
- A proteomic analysis of acute leukemia cells treated with 9-hydroxyoctadecadienoic acid.Lipids in health and disease · 2016Article
- The Nuts and Bolts of Transcriptionally Silent Chromatin in Saccharomyces cerevisiae.Genetics · 2016Review
- Dissecting the behavior and function of MBD3 in DNA methylation homeostasis by single-molecule spectroscopy and microscopy.Nucleic acids research · 2015Article
- Cohesin codes - interpreting chromatin architecture and the many facets of cohesin function.Journal of cell science · 2013Review
- Redefining the impact of nutrition on breast cancer incidence: is epigenetics involved?Nutrition research reviews · 2012Review
- Clamp loader ATPases and the evolution of DNA replication machinery.BMC biology · 2012Article
- Linking DNA replication to heterochromatin silencing and epigenetic inheritance.Acta biochimica et biophysica Sinica · 2012Review
- Inside single cells: quantitative analysis with advanced optics and nanomaterials.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnologyReview
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
In Saccharomyces cerevisiae, silent chromatin is formed at HMR upon the passage through S phase, yet neither the initiation of DNA replication at silencers nor the passage of a replication fork through HMR is required for silencing. Paradoxically, mutations in the DNA replication processivity factor, POL30, disrupt silencing despite this lack of requirement for DNA replication in the establishment of silencing. We tested whether pol30 mutants could establish silencing at either replicated or non-replicated HMR loci during S phase and found that pol30 mutants were defective in establishing silencing at HMR regardless of its replication status. Although previous studies tie the silencing defect of pol30 mutants to the chromatin assembly factors Asf1p and CAF-1, we found pol30 mutants did not exhibit a gross defect in packaging HMR into chromatin. Rather, the pol30 mutants exhibited defects in histone modifications linked to ASF1 and CAF-1-dependent pathways, including SAS-I- and Rtt109p-dependent acetylation events at H4-K16 and H3-K9 (plus H3-K56; Miller, A., Yang, B., Foster, T., and Kirchmaier, A. L. (2008) Genetics 179, 793-809). Additional experiments using FLIM-FRET revealed that Pol30p interacted with SAS-I and Rtt109p in the nuclei of living cells. However, these interactions were disrupted in pol30 mutants with defects linked to ASF1- and CAF-1-dependent pathways. Together, these results imply that Pol30p affects epigenetic processes by influencing the composition of chromosomal histone modifications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.