ArticleNucleic acids research2011
An insulator embedded in the chicken α-globin locus regulates chromatin domain configuration and differential gene expression.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 31 citations in OpenAlex.
- CTCF delimits a DNA methylation transition at the intergenic region between the embryonic and adult α-globin genes.Epigenetics · 2026Article
- A cross-sectional study on the assessment of COLL11A1, VEGF, and GDF5 gene polymorphisms in Turkish patients with primary knee osteoarthritis.BMC musculoskeletal disorders · 2025Article
- Boundary sequences flanking the mouse tyrosinase locus ensure faithful pattern of gene expression.Scientific reports · 2020Article
- Hypertrophic chondrocyte-specificAmerican journal of translational research · 2019Article
- Developing in 3D: the role of CTCF in cell differentiation.Development (Cambridge, England) · 2018Review
- CTCF as a multifunctional protein in genome regulation and gene expression.Experimental & molecular medicine · 2015Review
- Functional validation of mouse tyrosinase non-coding regulatory DNA elements by CRISPR-Cas9-mediated mutagenesis.Nucleic acids research · 2015Article
- A long non-coding RNA promotes full activation of adult gene expression in the chicken α-globin domain.Epigenetics · 2014Article
- CTCF demarcates chicken embryonic α-globin gene autonomous silencing and contributes to adult stage-specific gene expression.Epigenetics · 2013Article
- Allelic expression analysis of the osteoarthritis susceptibility gene COL11A1 in human joint tissues.BMC musculoskeletal disorders · 2013Article
- A role for insulator elements in the regulation of gene expression response to hypoxia.Nucleic acids research · 2012Article
- In the loop: long range chromatin interactions and gene regulation.Briefings in functional genomics · 2011Review
- Regulatory Elements in Vectors for Efficient Generation of Cell Lines Producing Target Proteins.Acta naturaeArticle
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genome organization into transcriptionally active domains denotes one of the first levels of gene expression regulation. Although the chromatin domain concept is generally accepted, only little is known on how domain organization impacts the regulation of differential gene expression. Insulators might hold answers to address this issue as they delimit and organize chromatin domains. We have previously identified a CTCF-dependent insulator with enhancer-blocking activity embedded in the 5' non-coding region of the chicken α-globin domain. Here, we demonstrate that this element, called the αEHS-1.4 insulator, protects a transgene against chromosomal position effects in stably transfected cell lines and transgenic mice. We found that this insulator can create a regulated chromatin environment that coincides with the onset of adult α-globin gene expression. Furthermore, such activity is in part dependent on the in vivo regulated occupancy of CTCF at the αEHS-1.4 element. Insulator function is also regulated by CTCF poly(ADP-ribosyl)ation. Our results suggest that the αEHS-1.4 insulator contributes in organizing the chromatin structure of the α-globin gene domain and prevents activation of adult α-globin gene expression at the erythroblast stage via CTCF.
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