ReviewBiology of the cell2010
Reverse genetics in eukaryotes.
Review in Biology of the cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 68 citations in OpenAlex.
- Evolution-guided yeast complementation reveals functional differences in human PSPH variants.FEBS open bio · 2026Article
- Host-Pathogen Interaction Interface: Promising Candidate Targets for Vaccine-Induced Protective and Memory Immune Responses.Vaccines · 2025Review
- Unlocking biological mechanisms with integrative functional genomics approaches.Molecules and cells · 2024Review
- Antisense Oligonucleotide Activation via Enzymatic Antibiotic Resistance Mechanism.ACS chemical biology · 2023Article
- The history and conceptual framework of assays and screens.BioEssays : news and reviews in molecular, cellular and developmental biology · 2023Article
- Genetic Manipulation of Haloferax Species.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Anti-Tick Vaccines: Current Advances and Future Prospects.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Recent advances in rotavirus reverse genetics and its utilization in basic research and vaccine development.Archives of virology · 2021Review
- Yippee like 4 (Ypel4) is essential for normal mouse red blood cell membrane integrity.Scientific reports · 2021Article
- Identifying adaptive alleles in the human genome: from selection mapping to functional validation.Human genetics · 2021Review
- Applications ofStem cells international · 2021Review
- Reverse Genetics for Peste des Petits Ruminants Virus: Current Status and Lessons to Learn from Other Non-segmented Negative-Sense RNA Viruses.Virologica Sinica · 2018Review
- Arrayed mutant haploid embryonic stem cell libraries facilitate phenotype-driven genetic screens.Nucleic acids research · 2017Article
- Modeling congenital disease and inborn errors of development in Drosophila melanogaster.Disease models & mechanisms · 2016Review
- The short-lived African turquoise killifish: an emerging experimental model for ageing.Disease models & mechanisms · 2016Review
- Ecotoxicogenomic approaches for understanding molecular mechanisms of environmental chemical toxicity using aquatic invertebrate, Daphnia model organism.International journal of molecular sciences · 2015Review
- Bacterial cellular engineering by genome editing and gene silencing.International journal of molecular sciences · 2014Review
- Going forward with genetics: recent technological advances and forward genetics in mice.The American journal of pathology · 2013Review
- Growth of transplastomic cells expressing D-amino acid oxidase in chloroplasts is tolerant to D-alanine and inhibited by D-valine.Plant physiology · 2012Article
- Tandem fluorescent protein timers for in vivo analysis of protein dynamics.Nature biotechnology · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reverse genetics consists in the modification of the activity of a target gene to analyse the phenotypic consequences. Four main approaches are used towards this goal and will be explained in this review. Two of them are centred on genome alterations. Mutations produced by random chemical or insertional mutagenesis can be screened to recover only mutants in a specific gene of interest. Alternatively, these alterations may be specifically targeted on a gene of interest by HR (homologous recombination). The other two approaches are centred on mRNA. RNA interference is a powerful method to reduce the level of gene products, while MO (morpholino) antisense oligonucleotides alter mRNA metabolism or translation. Some model species, such as Drosophila, are amenable to most of these approaches, whereas other model species are restricted to one of them. For example, in mice and yeasts, gene targeting by HR is prevalent, whereas in Xenopus and zebrafish MO oligonucleotides are mainly used. Genome-wide collections of mutants or inactivated models obtained in several species by these approaches have been made and will help decipher gene functions in the post-genomic era.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.