ArticleBiotechnology and bioengineering2011
Cellular arrays for large-scale analysis of transcription factor activity.
Article in Biotechnology and bioengineering, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 23 citations in OpenAlex.
- Single-cell transcriptomic analysis deciphers heterogeneity and transcriptional regulatory programs of sepsis with different prognosis.Biology direct · 2026Article
- The use of RNA-based treatments in the field of cancer immunotherapy.Molecular cancer · 2023Review
- Extracellular Matrix Proteins Confer Cell Adhesion-Mediated Drug Resistance Through Integrin αFrontiers in cell and developmental biology · 2021Article
- Towards systems tissue engineering: Elucidating the dynamics, spatial coordination, and individual cells driving emergent behaviors.Biomaterials · 2020Review
- Metabolic profiling of cancer cells reveals genome-wide crosstalk between transcriptional regulators and metabolism.Nature communications · 2019Article
- Dynamic microRNA activity identifies therapeutic targets in trastuzumab-resistant HER2Biotechnology and bioengineering · 2018Article
- Design of Large-Scale Reporter Construct Arrays for Dynamic, Live Cell Systems Biology.ACS synthetic biology · 2018Article
- Embryonic stem cell secreted factors decrease invasiveness of triple-negative breast cancer cells through regulome modulation.Cancer biology & therapy · 2018Article
- Fibronectin Promotes the Malignancy of Glioma Stem-Like Cells Via Modulation of Cell Adhesion, Differentiation, Proliferation and Chemoresistance.Frontiers in molecular neuroscience · 2018Article
- Synergistic effect of eribulin and CDK inhibition for the treatment of triple negative breast cancer.Oncotarget · 2017Article
- Simultaneous Pathway Activity Inference and Gene Expression Analysis Using RNA Sequencing.Cell systems · 2016Article
- Article
- Lentiviral arrays for live-cell dynamic monitoring of gene and pathway activity during stem cell differentiation.Molecular therapy : the journal of the American Society of Gene Therapy · 2014Article
- Dynamic transcription factor activity profiles reveal key regulatory interactions during megakaryocytic and erythroid differentiation.Biotechnology and bioengineering · 2014Article
- Inhibition of CDK-mediated phosphorylation of Smad3 results in decreased oncogenesis in triple negative breast cancer cells.Cell cycle (Georgetown, Tex.) · 2014Article
- Transcription factor networks in invasion-promoting breast carcinoma-associated fibroblasts.Cancer microenvironment : official journal of the International Cancer Microenvironment Society · 2013Article
- Dynamic transcription factor activity profiling in 2D and 3D cell cultures.Biotechnology and bioengineering · 2013Article
- The impact of adhesion peptides within hydrogels on the phenotype and signaling of normal and cancerous mammary epithelial cells.Biomaterials · 2012Article
- The living microarray: a high-throughput platform for measuring transcription dynamics in single cells.BMC genomics · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
Identifying molecular mechanisms or therapeutic targets is typically based on large-scale cellular analysis that measures the abundance of mRNA or protein; however, abundance does not necessarily correlate with activity. We report a method for direct large-scale quantification of active pathways that employs a cellular array with parallel gene delivery of constructs that report pathway activity. The reporter constructs encode luciferase, whose expression is influenced by binding of transcription factors (TFs), which are the downstream targets of signaling pathways. Luciferase levels are quantified by bioluminescence imaging (BLI), which allows for rapid, non-invasive measurements. Activity profiles by BLI of 32 TFs were robust, consistent, and reproducible, and correlated with standard cell lysis techniques. The array identified five TFs with differential activity during phorbol-12-myristate-13-acetate (PMA)-induced differentiation of breast cancer cells. A system for rapid, large-scale, BLI quantification of pathway activity provides an enabling technology for mechanistic studies of cellular responses and processes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.