ArticlePlastic and reconstructive surgery2010
Paracrine interaction between adipose-derived stromal cells and cranial suture-derived mesenchymal cells.
Article in Plastic and reconstructive surgery, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 17 citations in OpenAlex.
- Aloe polysaccharide promotes osteogenesis potential of adipose-derived stromal cells via BMP-2/Smads and prevents ovariectomized-induced osteoporosis.Molecular biology reports · 2022Article
- Coordinating Tissue Regeneration Through Transforming Growth Factor-β Activated Kinase 1 Inactivation and Reactivation.Stem cells (Dayton, Ohio) · 2019Article
- Jawbone microenvironment promotes periodontium regeneration by regulating the function of periodontal ligament stem cells.Scientific reports · 2017Article
- Calvarial Defect Healing Induced by Small Molecule Smoothened Agonist.Tissue engineering. Part A · 2016Article
- Autocrine Sonic hedgehog signaling promotes gastric cancer proliferation through induction of phospholipase Cγ1 and the ERK1/2 pathway.Journal of experimental & clinical cancer research : CR · 2016Article
- Article
- Adipose-derived mesenchymal stem cells from ventral hernia repair patients demonstrate decreased vasculogenesis.BioMed research international · 2014Article
- Exploiting paracrine mechanisms of tissue regeneration to repair damaged organs.Transplantation research · 2013Article
- Additive effects of sonic hedgehog and Nell-1 signaling in osteogenic versus adipogenic differentiation of human adipose-derived stromal cells.Stem cells and development · 2012Article
- Enhancement of human adipose-derived stromal cell angiogenesis through knockdown of a BMP-2 inhibitor.Plastic and reconstructive surgery · 2012Article
- Dura mater stimulates human adipose-derived stromal cells to undergo bone formation in mouse calvarial defects.Stem cells (Dayton, Ohio) · 2011Article
- Human adipose-derived stromal cells stimulate autogenous skeletal repair via paracrine Hedgehog signaling with calvarial osteoblasts.Stem cells and development · 2011Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundAdipose-derived stromal cells are a potential cell source for the successful healing of skeletal defects. In this study, the authors sought to investigate the potential for cranial suture-derived mesenchymal cells to promote the osteogenic differentiation of adipose-derived stromal cells. Various reports have previously examined the unique in vitro attributes of suture-derived mesenchymal cells; this study sought to extend those findings.
methodsSuture-derived mesenchymal cells were isolated from wild-type mice (n = 30) from both fusing posterofrontal and patent sagittal sutures. Cells were placed in Transwell inserts with human adipose-derived stromal cells (n = 5 patients) with osteogenic differentiation medium with or without recombinant Noggin (10 to 400 ng/ml). Specific gene expression of osteogenic markers and Hedgehog pathway were assayed; standard osteogenic assays (alkaline phosphatase and alizarin red staining) were performed. All assays were performed in triplicate.
resultsBoth posterofrontal and sagittal suture-derived mesenchymal cells induced osteogenic differentiation of adipose-derived stromal cells (p < 0.05). Posterofrontal suture-derived mesenchymal cells induced adipose-derived stromal cell osteogenesis to a greater degree than sagittal suture-derived mesenchymal cells (p < 0.05). This was accompanied by an increase in bone morphogenetic protein expression (p < 0.05). Finally, recombinant Noggin mitigated the pro-osteogenic effects of co-culture accompanied by a reduction in Hedgehog signaling (p < 0.05).
conclusionsSuture-derived mesenchymal cells secrete paracrine factors that induce osteogenic differentiation of multipotent stromal cells (human adipose-derived stromal cells). Cells derived from the fusing posterofrontal suture do this to a significantly greater degree than cells from the patent sagittal suture. Enhanced bone morphogenetic protein and Hedgehog signaling may underlie this paracrine effect.
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