ArticleProceedings of the National Academy of Sciences of the United States of America2010
A quantitative model of glucose signaling in yeast reveals an incoherent feed forward loop leading to a specific, transient pulse of transcription.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 41 citations in OpenAlex.
- The GATA8-GRF5-XTH9 feed-forward loop regulates cell size in poplar.Horticulture research · 2026Article
- Dynamic patterns of gene expression match extracellular signals through push-pull regulation.PLoS genetics · 2025Article
- Profiling proteomic responses to hexokinase-II depletion in terpene-producingEngineering microbiology · 2023Article
- Modelling of glucose repression signalling in yeast Saccharomyces cerevisiae.FEMS yeast research · 2022Review
- Genetic circuit design automation for yeast.Nature microbiology · 2020Article
- Transcriptomes of a xylose-utilizing industrial flocculating Saccharomyces cerevisiae strain cultured in media containing different sugar sources.AMB Express · 2016Article
- Model-based transcriptome engineering promotes a fermentative transcriptional state in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Innate immune memory and homeostasis may be conferred through crosstalk between the TLR3 and TLR7 pathways.Science signaling · 2016Article
- Helix-loop-helix/basic helix-loop-helix transcription factor network represses cell elongation in Arabidopsis through an apparent incoherent feed-forward loop.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Regulations of sugar transporters: insights from yeast.Current genetics · 2013Review
- An internal deletion in MTH1 enables growth on glucose of pyruvate-decarboxylase negative, non-fermentative Saccharomyces cerevisiae.Microbial cell factories · 2012Article
- Nutritional control of growth and development in yeast.Genetics · 2012Review
- Regulation of conditional gene expression by coupled transcription repression and RNA degradation.Nucleic acids research · 2012Article
- Thermodynamic state ensemble models of cis-regulation.PLoS computational biology · 2012Article
- Automated refinement and inference of analytical models for metabolic networks.Physical biology · 2011Article
- Cellular effects and epistasis among three determinants of adaptation in experimental populations of Saccharomyces cerevisiae.Eukaryotic cell · 2011Article
- An incoherent regulatory network architecture that orchestrates B cell diversification in response to antigen signaling.Molecular systems biology · 2011Article
- Gene regulatory networks and the role of robustness and stochasticity in the control of gene expression.Genome research · 2011Article
- The structure of dynamic GPCR signaling networks.Wiley interdisciplinary reviews. Systems biology and medicineReview
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The ability to design and engineer organisms demands the ability to predict kinetic responses of novel regulatory networks built from well-characterized biological components. Surprisingly, few validated kinetic models of complex regulatory networks have been derived by combining models of the network components. A major bottleneck in producing such models is the difficulty of measuring in vivo rate constants for components of complex networks. We demonstrate that a simple, genetic approach to measuring rate constants in vivo produces an accurate kinetic model of the complex network that Saccharomyces cerevisiae employs to regulate the expression of genes encoding glucose transporters. The model predicts a transient pulse of transcription of HXT4 (but not HXT2 or HXT3) in response to addition of a small amount of glucose to cells, an outcome we observed experimentally. Our model also provides a mechanistic explanation for this result: HXT2-4 are governed by a type 2, incoherent feed forward regulatory loop involving the Rgt1 and Mig2 transcriptional repressors. The efficiency with which Rgt1 and Mig2 repress expression of each HXT gene determines which of them have a pulse of transcription in response to glucose. Finally, the model correctly predicts how lesions in the feed forward loop change the kinetics of induction of HXT4 expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.