ArticleProceedings of the National Academy of Sciences of the United States of America2010
A genetic mosaic approach for neural circuit mapping in Drosophila.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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52 citing papers in PubMed, 92 citations in OpenAlex.
- Light-inducible FLPase reconstitution enables temporal control of gene expression in Drosophila melanogaster.Cell reports methods · 2026Article
- Integrator subunit IntS11 orchestrates the temporal dynamics of neural lineage progression in Drosophila.Cell & bioscience · 2026Article
- Tension transmission across a supracellular network drives increased tissue rigidity in the Drosophila retina.Cell reports · 2025Article
- Activity of nested neural circuits drives different courtship songs in Drosophila.Nature neuroscience · 2024Article
- Article
- Active antennal movements in Drosophila can tune wind encoding.Current biology : CB · 2023Article
- Serotonin Signaling Modulates Sexual Receptivity of Virgin Female Drosophila.Neuroscience bulletin · 2022Article
- A neurogenetic mechanism of experience-dependent suppression of aggression.Science advances · 2022Article
- Octopaminergic/tyraminergic Tdc2 neurons regulate biased sperm usage in female Drosophila melanogaster.Genetics · 2022Article
- The mitochondrial β-oxidation enzyme HADHA restrains hepatic glucagon response by promoting β-hydroxybutyrate production.Nature communications · 2022Article
- Central and Peripheral Clock Control of Circadian Feeding Rhythms.Journal of biological rhythms · 2021Article
- Germ cells commit somatic stem cells to differentiation following priming by PI3K/Tor activity in the Drosophila testis.PLoS genetics · 2021Article
- Analysis of growth cone extension in standardized coordinates highlights self-organization rules during wiring of the Drosophila visual system.PLoS genetics · 2021Article
- The role of Ire1 in Drosophila eye pigmentation revealed by an RNase dead allele.Developmental biology · 2021Article
- Accelerated cell cycles enable organ regeneration under developmental time constraints in the Drosophila hindgut.Developmental cell · 2021Article
- Visceral Mechano-sensing Neurons Control Drosophila Feeding by Using Piezo as a Sensor.Neuron · 2020Article
- A pair of ascending neurons in the subesophageal zone mediates aversive sensory inputs-evoked backward locomotion in Drosophila larvae.PLoS genetics · 2020Article
- SPARC enables genetic manipulation of precise proportions of cells.Nature neuroscience · 2020Article
- Article
- Recent Advances in the Genetic Dissection of Neural Circuits in Drosophila.Neuroscience bulletin · 2019Review
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Transgenic manipulation of subsets of brain cells is increasingly used for studying behaviors and their underlying neural circuits. In Drosophila, the GAL4-upstream activating sequence (UAS) binary system is powerful for gene manipulation, but GAL4 expression is often too broad for fine mapping of neural circuits. Here, we describe the development of unique molecular genetic tools to restrict GAL4 expression patterns. Building on the GAL4-UAS system, our method adds two components: a collection of enhancer-trap recombinase, Flippase (ET-FLP), transgenic lines that provide inheritable, reproducible, and tissue-specific FLP and an FRT-dependent GAL80 "flip-in" construct that converts FLP expression into tissue-specific repression of GAL4 by GAL80. By including a UAS-encoded fluorescent protein, circuit morphology can be simultaneously marked while the circuit function is assessed using another UAS transgene. In a proof-of-principle analysis, we applied this ET-FLP-induced intersectional GAL80/GAL4 repression (FINGR) method to map the neural circuitry underlying fly wing inflation. The FINGR system is versatile and powerful in combination with the vast collection of GAL4 lines for neural circuit mapping as well as for clonal analysis based on the infusion of the yeast-derived FRT/FLP system of mitotic recombination into Drosophila. The strategies and tactics underlying our FINGR system are also applicable to other genetically amenable organisms in which transgenes including the GAL4, UAS, GAL80, and FLP factors can be applied.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.