ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2010
Rapid delivery of internalized signaling receptors to the somatodendritic surface by sequence-specific local insertion.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 34 citations in OpenAlex.
- Cocaine-regulated trafficking of dopamine transporters in cultured neurons revealed by a pH sensitive reporter.iScience · 2023Article
- Functional independence of endogenous μ- and δ-opioid receptors co-expressed in cholinergic interneurons.eLife · 2021Article
- Opioid Pharmacology under the Microscope.Molecular pharmacology · 2020Review
- Article
- Temporal dependence of shifts in mu opioid receptor mobility at the cell surface after agonist binding observed by single-particle tracking.Scientific reports · 2019Article
- Imaging GPCRs trafficking and signaling with total internal reflection fluorescence microscopy in cultured neurons.Methods in cell biology · 2016Article
- Minireview: Spatial Programming of G Protein-Coupled Receptor Activity: Decoding Signaling in Health and Disease.Molecular endocrinology (Baltimore, Md.) · 2015Review
- Article
- Cell-autonomous regulation of Mu-opioid receptor recycling by substance P.Cell reports · 2015Article
- Opioid receptor trafficking and interaction in nociceptors.British journal of pharmacology · 2015Review
- Recent advances on the δ opioid receptor: from trafficking to function.British journal of pharmacology · 2015Review
- Visualizing and quantitating sequence-dependent GPCR recycling.Methods in cell biology · 2015Article
- Real-time imaging of mu opioid receptors by total internal reflection fluorescence microscopy.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Recycling endosomes undergo rapid closure of a fusion pore on exocytosis in neuronal dendrites.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Article
- Imaging of kiss-and-run exocytosis of surface receptors in neuronal cultures.Frontiers in cellular neuroscience · 2014Article
- Divergent modes for cargo-mediated control of clathrin-coated pit dynamics.Molecular biology of the cell · 2013Article
- Regulation of μ-opioid receptors: desensitization, phosphorylation, internalization, and tolerance.Pharmacological reviews · 2013Review
- Imaging pHluorin-tagged receptor insertion to the plasma membrane in primary cultured mouse neurons.Journal of visualized experiments : JoVE · 2012Article
- Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
The recycling pathway is a major route for delivering signaling receptors to the somatodendritic plasma membrane. We investigated the cell biological basis for the remarkable selectivity and speed of this process. We focused on the mu-opioid neuropeptide receptor and the beta(2)-adrenergic catecholamine receptor, two seven-transmembrane signaling receptors that traverse the recycling pathway efficiently after ligand-induced endocytosis and localize at steady state throughout the postsynaptic surface. Rapid recycling of each receptor in dissociated neuronal cultures was mediated by a receptor-specific cytoplasmic sorting sequence. Total internal reflection fluorescence microscopy imaging revealed that both sequences drive recycling via discrete vesicular fusion events in the cell body and dendritic shaft. Both sequences promoted recycling via "transient"-type events characterized by nearly immediate lateral spread of receptors after vesicular insertion resembling receptor insertion events observed previously in non-neural cells. The sequences differed in their abilities to produce distinct "persistent"-type events at which inserted receptors lingered for a variable time period before lateral spread. Both types of insertion event generated a uniform distribution of receptors in the somatodendritic plasma membrane when imaged over a 1 min interval, but persistent events uniquely generated a punctate surface distribution over a 10 s interval. These results establish sequence-directed recycling of signaling receptors in CNS neurons and show that this mechanism has the ability to generate receptor-specific patterns of local surface distribution on a timescale overlapping that of rapid physiological signaling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.