ArticleThe American journal of tropical medicine and hygiene2010
A recombinant AeDNA containing the insect-specific toxin, BmK IT1, displayed an increasing pathogenicity on Aedes albopictus.
Article in The American journal of tropical medicine and hygiene, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Synthesis of U-theraphotoxin-Pv1a_1, anToxicon: X · 2025Article
- Mosquito densovirus significantly reduces the vector susceptibility to dengue virus serotype 2 in Aedes albopictus mosquitoes (Diptera: Culicidae).Infectious diseases of poverty · 2023Article
- Perspectives of vector management in the control and elimination of vector-borne zoonoses.Frontiers in microbiology · 2023Review
- Vertical and Horizontal Transmission of Cell Fusing Agent Virus in Aedes aegypti.Applied and environmental microbiology · 2022Article
- Overview of paratransgenesis as a strategy to control pathogen transmission by insect vectors.Parasites & vectors · 2022Review
- Densovirus Oil Suspension Significantly Improves the Efficacy and Duration of Larvicidal Activity againstViruses · 2022Article
- Recombinant Mosquito Densovirus withToxins · 2022Article
- Article
- Development of large-scale mosquito densovirus production by in vivo methods.Parasites & vectors · 2019Article
- A Novel Densovirus Isolated From the Asian Tiger Mosquito Displays Varied Pathogenicity Depending on Its Host Species.Frontiers in microbiology · 2019Article
- Use of a Recombinant Mosquito Densovirus As a Gene Delivery Vector for the Functional Analysis of Genes in Mosquito Larvae.Journal of visualized experiments : JoVE · 2017Article
- Microbial control of arthropod-borne disease.Memorias do Instituto Oswaldo Cruz · 2017Review
- Development of non-defective recombinant densovirus vectors for microRNA delivery in the invasive vector mosquito, Aedes albopictus.Scientific reports · 2016Article
- Article
- Diverse Array of New Viral Sequences Identified in Worldwide Populations of the Asian Citrus Psyllid (Diaphorina citri) Using Viral Metagenomics.Journal of virology · 2015Article
- Fighting Arbovirus Transmission: Natural and Engineered Control of Vector Competence in Aedes Mosquitoes.Insects · 2015Review
- A viral over-expression system for the major malaria mosquito Anopheles gambiae.Scientific reports · 2014Article
- Article
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4 authors.
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Abstract
The Aedes aegypti densovirus (AeDNV) has previously shown potential in mosquito control. To improve its efficacy as a biopesticide, the gene for an excitatory insect-specific toxin from Buthus martensii Karsch (BmK IT1) was inserted into the AeDNV genome and cloned into pUCA plasmid. The coding sequence for green fluorescent protein was ligated to the C-terminus of the BmK IT1 gene as a screening marker. Recombinant and helper plasmids were cotransfected into C6/36 cells; wild-type viruses were the controls. The recombinant viruses were identified and quantified by real-time polymerase chain reaction and exposed to Ae. albopictus larvae for the evaluation of its bioinsecticidal activity. LT(50) and LD(50) bioassays showed that the recombinant AeDNV had stronger and faster pathogenic effects on Ae. albopictus than the wild-type virus. This is the first report on the recombinant AeDNA containing the insect-specific toxin, BmK IT1, which may be used to develop a novel type of insecticide.
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