ArticleThe American journal of tropical medicine and hygiene2010
Enhanced determination of Streptococcus pneumoniae serotypes associated with invasive disease in Laos by using a real-time polymerase chain reaction serotyping assay with cerebrospinal fluid.
Article in The American journal of tropical medicine and hygiene, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Serotype distribution of invasive pneumococcal disease from countries of the WHO Africa, Americas, Eastern Mediterranean, South-East Asia, and Western Pacific regions: a systematic literature review from 2010 to 2021.Frontiers in public health · 2024Pooled it
- Article
- Persistence of Vaccine Serotype Carriage and Differences in Pneumococcal Carriage by Laboratory Method and Sample Type in Indigenous Individuals in the Southwest United States.The Journal of infectious diseases · 2025Observational
- Evaluation strategies for measuring pneumococcal conjugate vaccine impact in low-resource settings.Expert review of vaccines · 2022Article
- Antimicrobial use and resistance data in human and animal sectors in the Lao PDR: evidence to inform policy.BMJ global health · 2021Article
- Management of Central Nervous System Infections, Vientiane, Laos, 2003-2011.Emerging infectious diseases · 2019Article
- Characterisation of Invasive Streptococcus pneumoniae Isolated from Cambodian Children between 2007 - 2012.PloS one · 2016Article
- Orientia, rickettsia, and leptospira pathogens as causes of CNS infections in Laos: a prospective study.The Lancet. Global health · 2015Article
- Comparison of a Real-Time Multiplex PCR and Sequetyping Assay for Pneumococcal Serotyping.PloS one · 2015Article
- Single-plex quantitative assays for the detection and quantification of most pneumococcal serotypes.PloS one · 2015Article
- Serotype distribution and antibiotic susceptibility of invasive Streptococcus pneumoniae isolates in patients aged 50 years or older in Thailand.Human vaccines & immunotherapeutics · 2014Article
- The use of dried cerebrospinal fluid filter paper spots as a substrate for PCR diagnosis of the aetiology of bacterial meningitis in the Lao PDR.Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases · 2013Article
- Causes of non-malarial fever in Laos: a prospective study.The Lancet. Global health · 2013Article
- Sequential triplex real-time PCR assay for detecting 21 pneumococcal capsular serotypes that account for a high global disease burden.Journal of clinical microbiology · 2013Article
- Fool's gold: Why imperfect reference tests are undermining the evaluation of novel diagnostics: a reevaluation of 5 diagnostic tests for leptospirosis.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2012Article
- A prospective assessment of the accuracy of commercial IgM ELISAs in diagnosis of Japanese encephalitis virus infections in patients with suspected central nervous system infections in Laos.The American journal of tropical medicine and hygiene · 2012Article
- Concurrent serotyping and genotyping of pneumococci by use of PCR and electrospray ionization mass spectrometry.Journal of clinical microbiology · 2012Article
- Molecular typing methods for outbreak detection and surveillance of invasive disease caused by Neisseria meningitidis, Haemophilus influenzae and Streptococcus pneumoniae, a review.Microbiology (Reading, England) · 2011Review
- Urine antibiotic activity in patients presenting to hospitals in Laos: implications for worsening antibiotic resistance.The American journal of tropical medicine and hygiene · 2011Article
- Response to Moore and others (2010) and Mehta (2010).The American journal of tropical medicine and hygiene · 2011Article
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Abstract
A prospective hospital-based study was undertaken to define the incidence of invasive pneumococcal disease (IPD) and circulating serotypes in Laos. Of 10,799 patients with hemocultures and 353 patients with cerebrospinal fluid samples, 0.21% and 5.4%, respectively, were positive for Streptococcus pneumoniae, giving a total of 35 IPD patients. We developed a real-time polymerase chain reaction to detect serotypes represented in the 13-valent pneumococcal vaccine. A blinded evaluation comparing serotype as defined by the Quellung reaction versus the polymerase chain reaction demonstrated 100% concordance. The most frequent serotype (n = 33 patients) was 1 (n = 6), followed by serotypes 5, 6A/B/C, 14, and 23F. Serotypes represented in the 7-valent polysaccharide-protein conjugate vaccine (PCV-7) infected 39% of patients, with 73% coverage for the PCV-10 and PCV-13 vaccines. Although the sample size is small, these data suggest that the PCV-7 vaccine may have relatively low efficacy in Laos. Further studies are urgently needed to guide pneumococcal vaccine policy in Laos.
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