ArticleJournal of clinical microbiology2010
Improved PCR methods for detection of African rabies and rabies-related lyssaviruses.
Article in Journal of clinical microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 65 citations in OpenAlex.
- A step forward in molecular diagnostics of lyssaviruses--results of a ring trial among European laboratories.PloS one · 2013Trial
- A rabies-related lyssavirus from aMicrobiology resource announcements · 2023Article
- Comparative Neutralization Activity of Commercial Rabies Immunoglobulin against Diverse Lyssaviruses.Vaccines · 2023Article
- Rabies Virus Seroprevalence among Dogs in Limpopo National Park and the Phylogenetic Analyses of Rabies Viruses in Mozambique.Pathogens (Basel, Switzerland) · 2022Article
- Rabies Prophylactic and Treatment Options: An In Vitro Study of siRNA- and Aptamer-Based Therapeutics.Viruses · 2021Review
- Review
- Multi-annual performance evaluation of laboratories in post-mortem diagnosis of animal rabies: Which techniques lead to the most reliable results in practice?PLoS neglected tropical diseases · 2021Article
- Lyssaviruses in Insectivorous Bats, South Africa, 2003-2018.Emerging infectious diseases · 2020Article
- Economic and feasibility comparison of the dRIT and DFA for decentralized rabies diagnosis in resource-limited settings: The use of Nigerian dog meat markets as a case study.PLoS neglected tropical diseases · 2020Article
- Evaluation of six TaqMan RT-rtPCR kits on two thermocyclers for the reliable detection of rabies virus RNA.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2019Article
- Reverse transcription recombinase polymerase amplification assay for rapid detection of canine associated rabies virus in Africa.PloS one · 2019Article
- Multi-site evaluation of the LN34 pan-lyssavirus real-time RT-PCR assay for post-mortem rabies diagnostics.PloS one · 2018Article
- Development of molecular confirmation tools for swift and easy rabies diagnostics.Virology journal · 2017Article
- Epidemiology of Rabies in Lesotho: The Importance of Routine Surveillance and Virus Characterization.Tropical medicine and infectious disease · 2017Article
- Evaluation of a TaqMan Array Card for Detection of Central Nervous System Infections.Journal of clinical microbiology · 2017Article
- Enhanced diagnosis of rabies and molecular evidence for the transboundary spread of the disease in Mozambique.Journal of the South African Veterinary Association · 2017Article
- New isolations of the rabies-related Mokola virus from South Africa.BMC veterinary research · 2017Article
- A Pan-Lyssavirus Taqman Real-Time RT-PCR Assay for the Detection of Highly Variable Rabies virus and Other Lyssaviruses.PLoS neglected tropical diseases · 2017Article
- Dual Combined Real-Time Reverse Transcription Polymerase Chain Reaction Assay for the Diagnosis of Lyssavirus Infection.PLoS neglected tropical diseases · 2016Article
- Serological Evidence of Lyssaviruses among Bats on Southwestern Indian Ocean Islands.PloS one · 2016Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Eleven different lyssavirus species, four of which occur on the African continent, are presently recognized. These viruses cause rabies, the burden of which is highest in the developing world, where routine laboratory diagnosis is often not available. From an epidemiological and control perspective, it is necessary that diagnostic methods detect the diversity of lyssaviruses present in different regions of the world. A published and widely used heminested reverse transcription-PCR (hnRT-PCR) was evaluated for its ability to detect a panel of diverse African lyssaviruses. Due to the limitations experienced for this assay, an alternative hnRT-PCR was developed. The new assay was found to be accurate and sensitive in the detection of African lyssavirus RNA in a variety of clinical specimens. The assay was further adapted to a real-time PCR platform to allow rapid, one-step, quantitative, and single-probe detection, and an internal control for the verification of sample preparation was included. The limit of detection of the real-time PCR assay was 10 RNA copies per reaction, with inter- and intra-assay variability below 4%. Subsequently, in demonstrating utility, both assays were successfully applied to antemortem rabies diagnosis in humans. We believe that the quantitative real-time PCR assay could find application as a routine confirmatory test for rabies diagnosis in the future and that it will serve as a valuable research tool in the biology of African lyssaviruses. Alternatively, the hnRT-PCR assay can be used in laboratories that do not have access to expensive real-time PCR equipment for sensitive diagnosis of lyssaviruses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.