ArticleJournal of clinical microbiology2010
Impact of long-term storage on stability of standard DNA for nucleic acid-based methods.
Article in Journal of clinical microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Differences in the subgingival microbial population of chronic periodontitis in subjects with and without type 2 diabetes mellitus-a systematic review.Clinical oral investigations · 2018Pooled it
- How long should we keep clinical isolates involved in bone and joint infections in microbiology laboratories?European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Review
- Review
- Identification and validation of anoikis-related differentially expressed genes in nasopharyngeal carcinoma.Translational cancer research · 2025Article
- Development and validation of a 66K SNP array for the hard clam (Mercenaria mercenaria).BMC genomics · 2024Article
- Monochrome Multiplex Quantitative PCR Telomere Length Measurement.Journal of visualized experiments : JoVE · 2024Article
- Yield Gel via Quantitative Gel Electrophoresis.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Long-Range Polymerase Chain Reaction.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Quantitative PCR of Alu Repeats Using PowerUp™ SYBRMethods in molecular biology (Clifton, N.J.) · 2023Article
- Pasteurization, storage conditions and viral concentration methods influence RT-qPCR detection of SARS-CoV-2 RNA in wastewater.The Science of the total environment · 2022Article
- Long-term stabilization of DNA at room temperature using a one-step microwave assisted process.Emergent materials · 2022Article
- Efficient molecular encoding in multifunctional self-immolative urethanes.Cell reports. Physical science · 2021Article
- Multiple freeze-thaw cycles lead to a loss of consistency in poly(A)-enriched RNA sequencing.BMC genomics · 2021Article
- SARS-CoV-2 was already spreading in France in late December 2019.International journal of antimicrobial agents · 2020Article
- Utilization of Digital PCR in Quantity Verification of Plasmid Standards Used in Quantitative PCR.Frontiers in molecular biosciences · 2020Article
- Article
- Optimization of Chelex 100 resin-based extraction of genomic DNA from dried blood spots.Biology methods & protocols · 2020Article
- Effect of Staple Age on DNA Origami Nanostructure Assembly and Stability.Molecules (Basel, Switzerland) · 2019Article
- An alternative storage method for characterization of the intestinal microbiota through next generation sequencing.Revista do Instituto de Medicina Tropical de Sao Paulo · 2018Article
- The Development of an Effective Bacterial Single-Cell Lysis Method Suitable for Whole Genome Amplification in Microfluidic Platforms.Micromachines · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Real-time PCR is dependent upon a calibration function for quantification. While long-term storage of standards saves cost and time, solutions of DNA are prone to degradation. We present here the benchmark treatment for preservation of DNA standards, involving storage in 50% glycerol-double-distilled water, whereby a deviation of 0.2 threshold cycle (C(T)) values resulted after 100 days of storage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.