ArticleJournal of clinical microbiology2010
secA1 gene sequence polymorphisms for species identification of Nocardia species and recognition of intraspecies genetic diversity.
Article in Journal of clinical microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Clinical patterns, species-specific correlations, and therapeutic outcomes of nocardiosis in immunocompetent individuals: a systematic analysis based on literature.Annals of clinical microbiology and antimicrobials · 2026Pooled it
- Population-based microbiological characterization ofMicrobiology spectrum · 2025Article
- Genomic characteristics, virulence potential, antimicrobial resistance profiles, and phylogenetic insights into Nocardia cyriacigeorgica.Annals of clinical microbiology and antimicrobials · 2025Article
- Application of MALDI-TOF mass spectrometry for identification of Nocardia species.BMC microbiology · 2024Article
- Rapid, Easy, and Reliable Identification ofInternational journal of molecular sciences · 2023Article
- Updated Review onClinical microbiology reviews · 2022Review
- Epidemiology ofAntibiotics (Basel, Switzerland) · 2022Article
- Whole genome sequencing reveals the genomic diversity, taxonomic classification, and evolutionary relationships of the genus Nocardia.PLoS neglected tropical diseases · 2021Article
- Invasive Nocardiosis: Disease Presentation, Diagnosis and Treatment - Old Questions, New Answers?Infection and drug resistance · 2020Review
- The Complexities of Nocardia Taxonomy and Identification.Journal of clinical microbiology · 2018Review
- Nocardiosis in the Tropical Northern Territory of Australia, 1997-2014.Open forum infectious diseases · 2016Article
- Article
- Nocardia Septic Arthritis Complicating an Anterior Cruciate Ligament Repair.Journal of clinical microbiology · 2015Article
- Necrotizing pneumonia and empyema in an immunocompetent patient caused by Nocardia cyriacigeorgica and identified by 16S rRNA and secA1 sequencing.Annals of laboratory medicine · 2014Article
- Identification, typing, and phylogenetic relationships of the main clinical Nocardia species in spain according to their gyrB and rpoB genes.Journal of clinical microbiology · 2013Article
- Molecular identification of nocardia isolates from clinical samples and an overview of human nocardiosis in Brazil.PLoS neglected tropical diseases · 2013Article
- A PCR-based intergenic spacer region-capillary gel electrophoresis typing method for identification and subtyping of Nocardia species.Journal of clinical microbiology · 2012Article
- Nocardia neocaledoniensis [corrected] as a cause of skin and soft tissue infection.Journal of clinical microbiology · 2012Article
- Sulfonamide resistance in isolates of Nocardia spp. from a US multicenter survey.Journal of clinical microbiology · 2012Article
- Nocardia infections of the face and neck.Current infectious disease reports · 2011Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sequence analysis of the Nocardia essential secretory protein SecA1 gene (secA1) for species identification of 120 American Type Culture Collection (ATCC) and clinical isolates of Nocardia (16 species) was studied in comparison with 5'-end 606-bp 16S rRNA gene sequencing. Species determination by both methods was concordant for all 10 ATCC strains. secA1 gene sequencing provided the same species identification as 16S rRNA gene analysis for 94/110 (85.5%) clinical isolates. However, 40 (42.6%) isolates had sequences with <99.0% similarity to archived secA1 sequences for the species, including 29 Nocardia cyriacigeorgica (96.6 to 98.9% similarity) and 4 Nocardia veterana (91.5 to 98.9% similarity) strains. Discrepant species identification was obtained for 16 (14.5%) clinical isolates, including 13/23 Nocardia nova strains (identified as various Nocardia species by secA1 sequencing) and 1 isolate each of Nocardia abscessus (identified as Nocardia asiatica), Nocardia elegans (Nocardia africana), and Nocardia transvalensis (Nocardia blacklockiae); both secA1 gene sequence analysis and deduced amino acid sequence analysis determined the species to be different from those assigned by 16S rRNA gene sequencing. The secA1 locus showed high sequence diversity (66 sequence or genetic types versus 40 16S rRNA gene sequence types), which was highest for N. nova (14 secA1 sequence types), followed by Nocardia farcinica and N. veterana (n = 7 each); there was only a single sequence type among eight Nocardia paucivorans strains. The secA1 locus has potential for species identification as an adjunct to 16S rRNA gene sequencing but requires additional deduced amino acid sequence analysis. It may be a suitable marker for phylogenetic/subtyping studies.
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