ArticleThe Journal of biological chemistry2010
A plasma membrane wound proteome: reversible externalization of intracellular proteins following reparable mechanical damage.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 34 citations in OpenAlex.
- Endoplasmic Reticulum Redoxome: Protein Folding and Beyond.Biochemistry · 2026Review
- Structure and function of vimentin in the generation and secretion of extracellular vimentin in response to inflammation.Cell communication and signaling : CCS · 2025Review
- Pathophysiological Role of Vimentin Intermediate Filaments in Lung Diseases.Frontiers in cell and developmental biology · 2022Review
- Actin Cytoskeletal Dynamics in Single-Cell Wound Repair.International journal of molecular sciences · 2021Review
- ATG9A protects the plasma membrane from programmed and incidental permeabilization.Nature cell biology · 2021Article
- Insights into the role of ERp57 in cancer.Journal of Cancer · 2021Review
- CaCells · 2020Review
- Extracellular vimentin modulates human dendritic cell activation.Molecular immunology · 2018Article
- Control of cytoskeletal dynamics during cellular responses to pore forming toxins.Communicative & integrative biology · 2017Article
- High-Throughput Microplate-Based Assay to Monitor Plasma Membrane Wounding and Repair.Frontiers in cellular and infection microbiology · 2017Article
- Cell repair: Revisiting the patch hypothesis.Communicative & integrative biology · 2016Article
- Cell healing: Calcium, repair and regeneration.Seminars in cell & developmental biology · 2015Review
- Proteomic analysis of human follicular fluid from fertile women.Clinical proteomics · 2015Article
- Myosin II does not contribute to wound repair in Dictyostelium cells.Biology open · 2014Article
- TRIM72 is required for effective repair of alveolar epithelial cell wounding.American journal of physiology. Lung cellular and molecular physiology · 2014Article
- Phosphatidylserine exposure during apoptosis reflects bidirectional trafficking between plasma membrane and cytoplasm.Cell death and differentiation · 2013Article
- Regulation and physiological roles of the calpain system in muscular disorders.Cardiovascular research · 2012Review
- Use of quantitative membrane proteomics identifies a novel role of mitochondria in healing injured muscles.The Journal of biological chemistry · 2012Article
- Cytoskeleton responses in wound repair.Cellular and molecular life sciences : CMLS · 2012Review
- Protein disulfide isomerase-associated 6 is an ATF6-inducible ER stress response protein that protects cardiac myocytes from ischemia/reperfusion-mediated cell death.Journal of molecular and cellular cardiology · 2012Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cells in mechanically active tissues undergo constant plasma membrane damage that must be repaired to allow survival. To identify wound-associated proteins, a cell-impermeant, thiol-reactive biotinylation reagent was used to label and subsequently isolate intracellular proteins that become exposed on the surface of cultured cells after plasma membrane damage induced by scraping from substratum or crushing with glass beads. Scrape-damaged cells survived injury and were capable of forming viable colonies. Proteins that were exposed to the cell surface were degraded or internalized a few seconds to several minutes after damage, except for vimentin, which was detectable on the cell surface for at least an hour after injury. Seven major biotinylated protein bands were identified on SDS-PAGE gels. Mass spectrometric studies identified cytoskeletal proteins (caldesmon-1 and vimentin), endoplasmic reticulum proteins (ERp57, ERp5, and HSP47), and nuclear proteins (lamin C, heterogeneous nuclear ribonucleoprotein F, and nucleophosmin-1) as major proteins exposed after injury. Although caldesmon was a major wound-associated protein in calpain small subunit knock-out fibroblasts, it was rapidly degraded in wild-type cells, probably by calpains. Lamin C exposure after wounding was most likely the consequence of nuclear envelope damage. These studies document major intracellular proteins associated with the cell surface of reversibly damaged somatic cells. The studies also show that externalization of some proteins reported to have physiologic or pathologic roles on the cell surface can occur in cells undergoing plasma membrane damage and subsequent repair.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.