ArticleJournal of assisted reproduction and genetics2010
Length difference between equine ZFX and ZFY genes and its application for molecular sex determination.
Article in Journal of assisted reproduction and genetics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 35 citations in OpenAlex.
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- Utility and Applicability of a Universal Set of Primers in Identifying the Sex of South and Southeast Asian Mammals.Zoological studies · 2019Article
- Y-chromosomal genes affecting male fertility: A review.Veterinary world · 2016Review
- High expression of Zinc-finger protein X-linked is associated with reduced E-cadherin expression and unfavorable prognosis in nasopharyngeal carcinoma.International journal of clinical and experimental pathology · 2015Article
- Sexually-dimorphic targeting of functionally-related genes in COPD.BMC systems biology · 2014Article
- A quantitative real-time PCR method using an X-linked gene for sex typing in pigs.Molecular biotechnology · 2013Article
- Study of methylation of histone H3 lysine 9 and H3 lysine 27 during X chromosome inactivation in three types of cells.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2012Article
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Authors and funding
5 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
purposewe analyzed the sex chromosome-encoding ZFX-ZFY genes and tested molecular sexing using the amplification patterns of intron 9 of ZFX-ZFY in the horse. METHODS AND
resultsthe amplification of the ZFX-ZFY produced two distinct patterns, reflecting sexual dimorphism based on a length difference between the X and Y chromosomes. The amplification products from foals showed two distinct bands: one was common to all foals and mares, indicating that this band was amplified from ZFX, while the other was specific to some foals, indicating that it was from ZFY. The result based on the PCR assay was identical to the results of amplification of the Y chromosome-specific SRY gene and those of investigations of the phenotypic gender in three different horse populations.
conclusionwe suggest that this PCR strategy for determining sexes by comparing the amplification patterns of ZFX-ZFY genes is a convenient and precise method for discriminating sexes in horses.
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