ArticleJournal of the Association for Research in Otolaryngology : JARO2010
Identification of the hair cell soma-1 antigen, HCS-1, as otoferlin.
Article in Journal of the Association for Research in Otolaryngology : JARO, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 53 citations in OpenAlex.
- Dominant and recessive ATOH1 variants cause distinct neurodevelopmental disorders with hearing loss.American journal of human genetics · 2026Article
- Zebrafish pou3f3b controls saccular/auditory development and marks non-neuronal cells that delaminate from the otic vesicle to promote neuroblast maturation.Developmental biology · 2026Article
- Development of the zebrafish anterior lateral line system is influenced by underlying cranial neural crest.Developmental biology · 2025Article
- Development of the zebrafish anterior lateral line system is influenced by underlying cranial neural crest.bioRxiv : the preprint server for biology · 2025Article
- Hair cell regeneration, reinnervation, and restoration of hearing thresholds in the avian hearing organ.Cell reports · 2024Article
- Cell-type identity of the avian utricle.Cell reports · 2022Article
- Macrophages Respond Rapidly to Ototoxic Injury of Lateral Line Hair Cells but Are Not Required for Hair Cell Regeneration.Frontiers in cellular neuroscience · 2020Article
- Article
- Genomic non-redundancy of the mir-183/96/182 cluster and its requirement for hair cell maintenance.Scientific reports · 2019Article
- Ectopic otoconial formation in the lagena of the pigeon inner ear.Biology open · 2018Article
- Transmission Disrupted: Modeling Auditory Synaptopathy in Zebrafish.Frontiers in cell and developmental biology · 2018Review
- Wnt9a Can Influence Cell Fates and Neural Connectivity across the Radial Axis of the Developing Cochlea.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Two cell populations participate in clearance of damaged hair cells from the sensory epithelia of the inner ear.Hearing research · 2017Review
- Delayed Otolith Development Does Not Impair Vestibular Circuit Formation in Zebrafish.Journal of the Association for Research in Otolaryngology : JARO · 2017Article
- Activity-Dependent Phosphorylation by CaMKIIδ Alters the CaFrontiers in synaptic neuroscience · 2017Article
- Tryptophan-rich basic protein (WRB) mediates insertion of the tail-anchored protein otoferlin and is required for hair cell exocytosis and hearing.The EMBO journal · 2016Article
- Pseudo-immortalization of postnatal cochlear progenitor cells yields a scalable cell line capable of transcriptionally regulating mature hair cell genes.Scientific reports · 2015Article
- Ribbon synapses in zebrafish hair cells.Hearing research · 2015Review
- Otoferlin deficiency in zebrafish results in defects in balance and hearing: rescue of the balance and hearing phenotype with full-length and truncated forms of mouse otoferlin.Molecular and cellular biology · 2015Article
- Numb is not a critical regulator of Notch-mediated cell fate decisions in the developing chick inner ear.Frontiers in cellular neuroscience · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
Abstract
Hair cells, the mechanosensitive receptor cells of the inner ear, are critical for our senses of hearing and balance. The small number of these receptor cells in the inner ear has impeded the identification and characterization of proteins important for hair cell function. The binding specificity of monoclonal antibodies provides a means for identifying hair cell-specific proteins and isolating them for further study. We have generated a monoclonal antibody, termed hair cell soma-1 (HCS-1), which specifically immunolabels hair cells in at least five vertebrate classes, including sharks and rays, bony fish, amphibians, birds, and mammals. We used HCS-1 to immunoprecipitate the cognate antigen and identified it as otoferlin, a member of the ferlin protein family. Mutations in otoferlin underlie DFNB9, a recessive, nonsyndromic form of prelingual deafness characterized as an auditory neuropathy. Using immunocytochemistry, we find that otoferlin is associated with the entire basolateral membrane of the hair cells and with vesicular structures distributed throughout most of the hair cell cytoplasm. Biochemical assays indicate that otoferlin is tightly associated with membranes, as it is not solubilized by alterations in calcium or salt concentrations. HCS-1 immunolabeling does not co-localize with ribeye, a constituent of synaptic ribbons, suggesting that otoferlin may, in addition to its proposed function in synaptic vesicle release, play additional roles in hair cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.