ArticleHistochemistry and cell biology2010
Lamellar body ultrastructure revisited: high-pressure freezing and cryo-electron microscopy of vitreous sections.
Article in Histochemistry and cell biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 32 citations in OpenAlex.
- Comparative electron microscopic visualization of the lung alveolar epithelial glycocalyx with different staining and labeling methods.Journal of anatomy · 2025Article
- The Significance of Lipids for the Absorption and Release of Oxygen in Biological Organisms.Advances in experimental medicine and biology · 2023Article
- Novel opportunities from bioimaging to understand the trafficking and maturation of intracellular pulmonary surfactant and its role in lung diseases.Frontiers in immunology · 2023Review
- A recipe for a good clinical pulmonary surfactant.Biomedical journal · 2022Review
- Myelin sheath and cyanobacterial thylakoids as concentric multilamellar structures with similar bioenergetic properties.Open biology · 2021Article
- Volume electron microscopy: analyzing the lung.Histochemistry and cell biology · 2021Review
- Post-correlation on-lamella cryo-CLEM reveals the membrane architecture of lamellar bodies.Communications biology · 2021Article
- On Top of the Alveolar Epithelium: Surfactant and the Glycocalyx.International journal of molecular sciences · 2020Review
- ATP is stored in lamellar bodies to activate vesicular P2XThe Journal of general physiology · 2018Article
- In Vitro Induction and In Vivo Engraftment of Lung Bud Tip Progenitor Cells Derived from Human Pluripotent Stem Cells.Stem cell reports · 2018Article
- Ilomastat, a synthetic inhibitor of MMPs, prevents lung injury induced by γ-ray irradiation in mice.Oncotarget · 2017Article
- Using electron microscopes to look into the lung.Histochemistry and cell biology · 2016Review
- Spectral phasor analysis of LAURDAN fluorescence in live A549 lung cells to study the hydration and time evolution of intracellular lamellar body-like structures.Biochimica et biophysica acta · 2016Article
- Pneumocytes Assemble Lung Surfactant as Highly Packed/Dehydrated States with Optimal Surface Activity.Biophysical journal · 2015Article
- The ommatidia of Arca noae: a three-tier structure with a central light-guiding element for the receptor cell.Histochemistry and cell biology · 2011Article
- Histochemistry and cell biology: the annual review 2010.Histochemistry and cell biology · 2011Review
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lamellar bodies are the storage sites for lung surfactant within type II alveolar epithelial cells. The structure-function models of lamellar bodies are based on microscopic analyses of chemically fixed tissue. Despite available alternative fixation methods that are less prone to artifacts, such as cryofixation by high-pressure freezing, the nature of the lung, being mostly air filled, makes it difficult to take advantage of these improved methods. In this paper, we propose a new approach and show for the first time the ultrastructure of intracellular lamellar bodies based on cryo-electron microscopy of vitreous sections in the range of nanometer resolution. Thus, unspoiled by chemical fixation, dehydration and contrasting agents, a close to native structure is revealed. Our approach uses perfluorocarbon to substitute the air in the alveoli. Lung tissue was subsequently high-pressure frozen, cryosectioned and observed in a cryo-electron microscope. The lamellar bodies clearly show a tight lamellar morphology. The periodicity of these lamellae was 7.3 nm. Lamellar bifurcations were observed in our cryosections. The technical approach described in this paper allows the examination of the native cellular ultrastructure of the surfactant system under near in vivo conditions, and therefore opens up prospectives for scrutinizing various theories of lamellar body biogenesis, exocytosis and recycling.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.