ArticlePloS one2010
Identification and characterization of an unusual class I myosin involved in vesicle traffic in Trypanosoma brucei.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed, 28 citations in OpenAlex.
- The actomyosin system is essential for the integrity of the endosomal system in bloodstream formeLife · 2024Article
- To the Surface and Back: Exo- and Endocytic Pathways inFrontiers in cell and developmental biology · 2021Review
- The Myosin Family of Mechanoenzymes: From Mechanisms to Therapeutic Approaches.Annual review of biochemistry · 2020Review
- Emerging Functions of Actins and Actin Binding Proteins in Trypanosomatids.Frontiers in cell and developmental biology · 2020Review
- Evolutionary analyses of myosin genes in trypanosomatids show a history of expansion, secondary losses and neofunctionalization.Scientific reports · 2018Article
- Lineage-specific proteins essential for endocytosis in trypanosomes.Journal of cell science · 2017Article
- Nicotinamide inhibits the lysosomal cathepsin b-like protease and kills African trypanosomes.The Journal of biological chemistry · 2013Article
- Myosin 1E coordinates actin assembly and cargo trafficking during clathrin-mediated endocytosis.Molecular biology of the cell · 2012Article
- Cytokinesis in bloodstream stage Trypanosoma brucei requires a family of katanins and spastin.PloS one · 2012Article
- Affinity is an important determinant of the anti-trypanosome activity of nanobodies.PLoS neglected tropical diseases · 2012Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Myosins are a multimember family of motor proteins with diverse functions in eukaryotic cells. African trypanosomes possess only two candidate myosins and thus represent a useful system for functional analysis of these motors. One of these candidates is an unusual class I myosin (TbMyo1) that is expressed at similar levels but organized differently during the life cycle of Trypanosoma brucei. This myosin localizes to the polarized endocytic pathway in bloodstream forms of the parasite. This organization is actin dependent. Knock down of TbMyo1 results in a significant reduction in endocytic activity, a cessation in cell division and eventually cell death. A striking morphological feature in these cells is an enlargement of the flagellar pocket, which is consistent with an imbalance in traffic to and from the surface. In contrast TbMyo1 is distributed throughout procyclic forms of the tsetse vector and a loss of approximately 90% of the protein has no obvious effects on growth or morphology. These results reveal a life cycle stage specific requirement for this myosin in essential endocytic traffic and represent the first description of the involvement of a motor protein in vesicle traffic in these parasites.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.