ArticlePLoS pathogens2010
Giardia cyst wall protein 1 is a lectin that binds to curled fibrils of the GalNAc homopolymer.
Article in PLoS pathogens, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 51 citations in OpenAlex.
- Pathogenicity and virulence ofVirulence · 2026Review
- Review
- Functionally-derivatized Nanoantibodies as novel tools for Giardia lamblia cyst detection.PLoS neglected tropical diseases · 2026Article
- β-Giardin as an Immunomagnetic Enrichment Target for Multi-Host Detection ofPathogens (Basel, Switzerland) · 2025Article
- Staging Encystation Progression inFrontiers in cell and developmental biology · 2021Article
- Interaction between human mucins and parasite glycoproteins: the role of lectins and glycosidases in colonization by intestinal protozoa.Revista do Instituto de Medicina Tropical de Sao Paulo · 2020Article
- The most abundant cyst wall proteins of Acanthamoeba castellanii are lectins that bind cellulose and localize to distinct structures in developing and mature cyst walls.PLoS neglected tropical diseases · 2019Article
- Cracking the Sugar Code by Mass Spectrometry : An Invited Perspective in Honor of Dr. Catherine E. Costello, Recipient of the 2017 ASMS Distinguished Contribution Award.Journal of the American Society for Mass Spectrometry · 2018Article
- Mass Cytometry for Detection of Silver at the Bacterial Single Cell Level.Frontiers in microbiology · 2017Article
- Cyst-Wall-Protein-1 is fundamental for Golgi-like organelle neogenesis and cyst-wall biosynthesis in Giardia lamblia.Nature communications · 2016Article
- Article
- Ethanol and isopropanol in concentrations present in hand sanitizers sharply reduce excystation of Giardia and Entamoeba and eliminate oral infectivity of Giardia cysts in gerbils.Antimicrobial agents and chemotherapy · 2015Article
- Sphingolipids, Lipid Rafts, and Giardial Encystation: The Show Must Go On.Current tropical medicine reports · 2015Article
- Interaction and assembly of two novel proteins in the spore wall of the microsporidian species Nosema bombycis and their roles in adherence to and infection of host cells.Infection and immunity · 2015Article
- Article
- Strategies to discover the structural components of cyst and oocyst walls.Eukaryotic cell · 2013Review
- Persistent G. lamblia impairs growth in a murine malnutrition model.The Journal of clinical investigation · 2013Article
- Characterization and separation of Cryptosporidium and Giardia cells using on-chip dielectrophoresis.Biomicrofluidics · 2012Article
- Interaction forces drive the environmental transmission of pathogenic protozoa.Applied and environmental microbiology · 2012Review
- A simple fibril and lectin model for cyst walls of Entamoeba and perhaps Giardia.Trends in parasitology · 2011Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The infectious and diagnostic stage of Giardia lamblia (also known as G. intestinalis or G. duodenalis) is the cyst. The Giardia cyst wall contains fibrils of a unique beta-1,3-linked N-acetylgalactosamine (GalNAc) homopolymer and at least three cyst wall proteins (CWPs) composed of Leu-rich repeats (CWP(LRR)) and a C-terminal conserved Cys-rich region (CWP(CRR)). Our goals were to dissect the structure of the cyst wall and determine how it is disrupted during excystation. The intact Giardia cyst wall is thin (approximately 400 nm), easily fractured by sonication, and impermeable to small molecules. Curled fibrils of the GalNAc homopolymer are restricted to a narrow plane and are coated with linear arrays of oval-shaped protein complex. In contrast, cyst walls of Giardia treated with hot alkali to deproteinate fibrils of the GalNAc homopolymer are thick (approximately 1.2 microm), resistant to sonication, and permeable. The deproteinated GalNAc homopolymer, which forms a loose lattice of curled fibrils, is bound by native CWP1 and CWP2, as well as by maltose-binding protein (MBP)-fusions containing the full-length CWP1 or CWP1(LRR). In contrast, neither MBP alone nor MBP fused to CWP1(CRR) bind to the GalNAc homopolymer. Recombinant CWP1 binds to the GalNAc homopolymer within secretory vesicles of Giardia encysting in vitro. Fibrils of the GalNAc homopolymer are exposed during excystation or by treatment of heat-killed cysts with chymotrypsin, while deproteinated fibrils of the GalNAc homopolymer are degraded by extracts of Giardia cysts but not trophozoites. These results show the Leu-rich repeat domain of CWP1 is a lectin that binds to curled fibrils of the GalNAc homopolymer. During excystation, host and Giardia proteases appear to degrade bound CWPs, exposing fibrils of the GalNAc homopolymer that are digested by a stage-specific glycohydrolase.
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