ArticlePloS one2010
Mechanical strain stabilizes reconstituted collagen fibrils against enzymatic degradation by mammalian collagenase matrix metalloproteinase 8 (MMP-8).
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 98 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
98 citing papers in PubMed, 1 synthesis or guideline pooled it, 193 citations in OpenAlex.
- Mechanosensing by the nucleus: From pathways to scaling relationships.The Journal of cell biology · 2017Pooled it
- Computational multiscale analysis of growth and remodeling in the ascending aorta of the Marfan mouse.Biomechanics and modeling in mechanobiology · 2026Article
- Article
- Targeting muscle fibrosis using non-specific collagenase injections destabilizes the basal lamina and induces matrix remodeling.Skeletal muscle · 2026Article
- Targeting the D93 cryptic collagen epitope alters integrin α2β1-dependent cellular migration and collagen remodeling in metastatic breast cancer.Scientific reports · 2026Article
- Biomechanical Variation of the Vessel Wall Along the Length of the Healthy Aorta-Linking Geometric, Flow-, and Pressure-Mediated Adaptations.Journal of biomechanical engineering · 2026Review
- Collagen Mechanics.Sub-cellular biochemistry · 2026Review
- Low-fatigue, adjustable pressure garments at 10, 20 and 30 mm Hg reduce scar thickness and improve pliability.PloS one · 2026Article
- A Mathematical Model of Wound Healing Incorporating Strain-Induced MSC Differentiation.Annals of biomedical engineering · 2026Article
- In tendons, differing physiological requirements lead to distinct patterns of MMP-1 degradation.Scientific reports · 2025Article
- Microstructural remodeling under single fiber tensional homeostasis recreates distinctive ex vivo mechanical behavior of arteries.Biomechanics and modeling in mechanobiology · 2025Article
- Loss of collagen content is localized near cartilage lesions on the day of injurious loading and intensified on day 12.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2025Article
- Physiological mechanical forces accelerate the degradation of bovine lung collagen fibers by bacterial collagenase.Scientific reports · 2024Article
- Residual Strain and Joint Pressurization Maintain Collagen Tension for On-Joint Lumbar Facet Capsular Ligaments.Journal of biomechanical engineering · 2024Article
- Strain-dependent dynamic re-alignment of collagen fibers in skeletal muscle extracellular matrix.Acta biomaterialia · 2024Article
- Alignment, cross linking, and beyond: a collagen architect's guide to the skeletal muscle extracellular matrix.American journal of physiology. Cell physiology · 2023Review
- The non-affine fiber network solver: A multiscale fiber network material model for finite-element analysis.Journal of the mechanical behavior of biomedical materials · 2023Article
- The extracellular matrix of dystrophic mouse diaphragm accounts for the majority of its passive stiffness and is resistant to collagenase digestion.Matrix biology plus · 2023Article
- Mechanochemistry of collagen.Acta biomaterialia · 2023Review
- The Lumbar Facet Capsular Ligament Becomes More Anisotropic and the Fibers Become Stiffer With Intervertebral Disc and Facet Joint Degeneration.Journal of biomechanical engineering · 2023Article
38 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundCollagen, a triple-helical, self-organizing protein, is the predominant structural protein in mammals. It is found in bone, ligament, tendon, cartilage, intervertebral disc, skin, blood vessel, and cornea. We have recently postulated that fibrillar collagens (and their complementary enzymes) comprise the basis of a smart structural system which appears to support the retention of molecules in fibrils which are under tensile mechanical strain. The theory suggests that the mechanisms which drive the preferential accumulation of collagen in loaded tissue operate at the molecular level and are not solely cell-driven. The concept reduces control of matrix morphology to an interaction between molecules and the most relevant, physical, and persistent signal: mechanical strain. METHODOLOGY/PRINCIPAL
findingsThe investigation was carried out in an environmentally-controlled microbioreactor in which reconstituted type I collagen micronetworks were gently strained between micropipettes. The strained micronetworks were exposed to active matrix metalloproteinase 8 (MMP-8) and relative degradation rates for loaded and unloaded fibrils were tracked simultaneously using label-free differential interference contrast (DIC) imaging. It was found that applied tensile mechanical strain significantly increased degradation time of loaded fibrils compared to unloaded, paired controls. In many cases, strained fibrils were detectable long after unstrained fibrils were degraded. CONCLUSIONS/SIGNIFICANCE: In this investigation we demonstrate for the first time that applied mechanical strain preferentially preserves collagen fibrils in the presence of a physiologically-important mammalian enzyme: MMP-8. These results have the potential to contribute to our understanding of many collagen matrix phenomena including development, adaptation, remodeling and disease. Additionally, tissue engineering could benefit from the ability to sculpt desired structures from physiologically compatible and mutable collagen.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.