ArticlePloS one2010
A critical analysis of Atoh7 (Math5) mRNA splicing in the developing mouse retina.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 16 citations in OpenAlex.
- Direct Comparison of Constitutive Rax-Cre Transgenic Drivers That Activate in the Mouse Embryonic Eye Field.Investigative ophthalmology & visual science · 2026Article
- Integral bHLH factor regulation of cell cycle exit and RGC differentiation.Developmental dynamics : an official publication of the American Association of Anatomists · 2018Article
- The dynamics of native Atoh7 protein expression during mouse retinal histogenesis, revealed with a new antibody.Gene expression patterns : GEP · 2018Article
- Gastrin induces parathyroid hormone-like hormone expression in gastric parietal cells.American journal of physiology. Gastrointestinal and liver physiology · 2017Article
- The effects of C5-substituted 2,4-diaminoquinazolines on selected transcript expression in spinal muscular atrophy cells.PloS one · 2017Article
- Genetics of Combined Pituitary Hormone Deficiency: Roadmap into the Genome Era.Endocrine reviews · 2016Review
- Notch signaling differentially regulates Atoh7 and Neurog2 in the distal mouse retina.Development (Cambridge, England) · 2014Article
- Heterochronic misexpression of Ascl1 in the Atoh7 retinal cell lineage blocks cell cycle exit.Molecular and cellular neurosciences · 2013Article
- Distinct neurogenic potential in the retinal margin and the pars plana of mammalian eye.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- ATOH7 mutations cause autosomal recessive persistent hyperplasia of the primary vitreous.Human molecular genetics · 2012Article
- Pushing the envelope of retinal ganglion cell genesis: context dependent function of Math5 (Atoh7).Developmental biology · 2012Article
- Math5 defines the ganglion cell competence state in a subpopulation of retinal progenitor cells exiting the cell cycle.Developmental biology · 2012Article
- Retraction. Alternative splicing produces high levels of noncoding isoforms of bHLH transcription factors during development.Genes & development · 2011Article
- Deletion of a remote enhancer near ATOH7 disrupts retinal neurogenesis, causing NCRNA disease.Nature neuroscience · 2011Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The Math5 (Atoh7) gene is transiently expressed during retinogenesis by progenitors exiting mitosis, and is essential for ganglion cell (RGC) development. Math5 contains a single exon, and its 1.7 kb mRNA encodes a 149-aa polypeptide. Mouse Math5 mutants have essentially no RGCs or optic nerves. Given the importance of this gene in retinal development, we thoroughly investigated the possibility of Math5 mRNA splicing by Northern blot, 3'RACE, RNase protection assays, and RT-PCR, using RNAs extracted from embryonic eyes and adult cerebellum, or transcribed in vitro from cDNA clones. Because Math5 mRNA contains an elevated G+C content, we used graded concentrations of betaine, an isostabilizing agent that disrupts secondary structure. Although approximately 10% of cerebellar Math5 RNAs are spliced, truncating the polypeptide, our results show few, if any, spliced Math5 transcripts exist in the developing retina (<1%). Rare deleted cDNAs do arise via RT-mediated RNA template switching in vitro, and are selectively amplified during PCR. These data differ starkly from a recent study (Kanadia and Cepko 2010), which concluded that the vast majority of Math5 and other bHLH transcripts are spliced to generate noncoding RNAs. Our findings clarify the architecture of the Math5 gene and its mechanism of action. These results have implications for all members of the bHLH gene family, for any gene that is alternatively spliced, and for the interpretation of all RT-PCR experiments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.