ReviewJournal of biomolecular techniques : JBT2010
Laser capture microdissection in the tissue biorepository.
Review in Journal of biomolecular techniques : JBT, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed.
- Infrared Laser Ablation and Capture of Biological Tissue.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Article
- NGLY1 mutations cause protein aggregation in human neurons.Cell reports · 2023Article
- Review
- Comparative transcriptomics reveals highly conserved regional programs between porcine and human colonic enteric nervous system.Communications biology · 2023Article
- Spatially resolved analysis of FFPE tissue proteomes by quantitative mass spectrometry.Nature protocols · 2020Article
- Proteomic approaches for characterizing renal cell carcinoma.Clinical proteomics · 2020Review
- A novel in vitro model of metastasis supporting passive shedding hypothesis from murine pancreatic cancer Panc-02.Cytotechnology · 2019Article
- Laser capture microdissection: techniques and applications in liver diseases.Hepatology international · 2019Review
- Laser Capture Microdissection and RNA-Seq Analysis: High Sensitivity Approaches to Explain Histopathological Heterogeneity in Human Glioblastoma FFPE Archived Tissues.Frontiers in oncology · 2019Article
- An Insight into the Difficulties in the Discovery of Specific Biomarkers of Limbal Stem Cells.International journal of molecular sciences · 2018Review
- Laser Capture Microdissection of Epithelium from a Wound Healing Model for MicroRNA Analysis.Methods in molecular biology (Clifton, N.J.) · 2018Article
- A novel ex vivo Huntington's disease model for studying GABAergic neurons and cell grafts by laser microdissection.PloS one · 2018Article
- CCL18 from tumor-cells promotes epithelial ovarian cancer metastasis via mTOR signaling pathway.Molecular carcinogenesis · 2016Article
- In Situ Sodium Bisulfite Modification of Genomic DNA from Microdissected Melanoma Paraffin-Embedded Archival Tissues.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Laser capture microdissection: Big data from small samples.Histology and histopathology · 2015Review
- Technologies for Single-Cell Isolation.International journal of molecular sciences · 2015Review
- RNA Isolation from Cell Specific Subpopulations Using Laser-capture Microdissection Combined with Rapid Immunolabeling.Journal of visualized experiments : JoVE · 2015Article
- Laser assisted microdissection, an efficient technique to understand tissue specific gene expression patterns and functional genomics in plants.Molecular biotechnology · 2015Review
- Application of molecular technologies for phosphoproteomic analysis of clinical samples.Oncogene · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An important need of many cancer research projects is the availability of high-quality, appropriately selected tissue. Tissue biorepositories are organized to collect, process, store, and distribute samples of tumor and normal tissue for further use in fundamental and translational cancer research. This, in turn, provides investigators with an invaluable resource of appropriately examined and characterized tissue specimens and linked patient information. Human tissues, in particular, tumor tissues, are complex structures composed of heterogeneous mixtures of morphologically and functionally distinct cell types. It is essential to analyze specific cell types to identify and define accurately the biologically important processes in pathologic lesions. Laser capture microdissection (LCM) is state-of-the-art technology that provides the scientific community with a rapid and reliable method to isolate a homogeneous population of cells from heterogeneous tissue specimens, thus providing investigators with the ability to analyze DNA, RNA, and protein accurately from pure populations of cells. This is particularly well-suited for tumor cell isolation, which can be captured from complex tissue samples. The combination of LCM and a tissue biorepository offers a comprehensive means by which researchers can use valuable human biospecimens and cutting-edge technology to facilitate basic, translational, and clinical research. This review provides an overview of LCM technology with an emphasis on the applications of LCM in the setting of a tissue biorepository, based on the author's extensive experience in LCM procedures acquired at Fox Chase Cancer Center and Hollings Cancer Center.
Indexed as
Identifiers
20808641PMC2922833What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.