ArticleThe Plant cell2010
The cytosolic tail dipeptide Ile-Met of the pea receptor BP80 is required for recycling from the prevacuole and for endocytosis.
Article in The Plant cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 46 citations in OpenAlex.
- Turnip mosaic virus co-opts the vacuolar sorting receptor VSR4 to promote viral genome replication in plants by targeting viral replication vesicles to the endosome.PLoS pathogens · 2022Article
- Plant pathogen effector utilizes host susceptibility factor NRL1 to degrade the immune regulator SWAP70.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Nanobody-triggered lockdown of VSRs reveals ligand reloading in the Golgi.Nature communications · 2018Article
- Vacuolar Trafficking Protein VPS38 Is Dispensable for Autophagy.Plant physiology · 2018Article
- Dimerization of the Vacuolar Receptors AtRMR1 and -2 from Arabidopsis thaliana Contributes to Their Localization in the trans-Golgi Network.International journal of molecular sciences · 2016Article
- pH Regulation by NHX-Type Antiporters Is Required for Receptor-Mediated Protein Trafficking to the Vacuole in Arabidopsis.The Plant cell · 2015Article
- Vacuolar Sorting Receptor-Mediated Trafficking of Soluble Vacuolar Proteins in Plant Cells.Plants (Basel, Switzerland) · 2014Review
- Trafficking of Vacuolar Sorting Receptors: New Data and New Problems.Plant physiology · 2014Article
- Golgi-dependent transport of vacuolar sorting receptors is regulated by COPII, AP1, and AP4 protein complexes in tobacco.The Plant cell · 2014Article
- Detection of the virulent form of AVR3a from Phytophthora infestans following artificial evolution of potato resistance gene R3a.PloS one · 2014Article
- Routes to and from the plasma membrane: bulk flow versus signal mediated endocytosis.Plant signaling & behavior · 2014Article
- Receptor-mediated transport of vacuolar proteins: a critical analysis and a new model.Protoplasma · 2014Article
- In vivo intracellular pH measurements in tobacco and Arabidopsis reveal an unexpected pH gradient in the endomembrane system.The Plant cell · 2013Article
- MTV1 and MTV4 encode plant-specific ENTH and ARF GAP proteins that mediate clathrin-dependent trafficking of vacuolar cargo from the trans-Golgi network.The Plant cell · 2013Article
- Development and properties of genetically encoded pH sensors in plants.Frontiers in plant science · 2013Article
- Trafficking of vacuolar proteins: the crucial role of Arabidopsis vacuolar protein sorting 29 in recycling vacuolar sorting receptor.The Plant cell · 2012Article
- Relocalization of late blight resistance protein R3a to endosomal compartments is associated with effector recognition and required for the immune response.The Plant cell · 2012Article
- Mechanisms and concepts paving the way towards a complete transport cycle of plant vacuolar sorting receptors.The Plant cell · 2012Review
- Article
- Isolation and proteomic analysis of the SYP61 compartment reveal its role in exocytic trafficking in Arabidopsis.Cell research · 2012Article
Corrections and comments
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Authors and funding
5 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pea (Pisum sativum) BP80 is a vacuolar sorting receptor for soluble proteins and has a cytosolic domain essential for its intracellular trafficking between the trans-Golgi network and the prevacuole. Based on mammalian knowledge, we introduced point mutations in the cytosolic region of the receptor and produced chimeras of green fluorescent protein fused to the transmembrane domain of pea BP80 along with the modified cytosolic tails. By analyzing the subcellular location of these chimera, we found that mutating Glu-604, Asp-616, or Glu-620 had mild effects, whereas mutating the Tyr motif partially redistributed the chimera to the plasma membrane. Replacing both Ile-608 and Met-609 by Ala (IMAA) led to a massive redistribution of fluorescence to the vacuole, indicating that recycling is impaired. When the chimera uses the alternative route, the IMAA mutation led to a massive accumulation at the plasma membrane. Using Arabidopsis thaliana plants expressing a fluorescent reporter with the full-length sequence of At VSR4, we demonstrated that the receptor undergoes brefeldin A-sensitive endocytosis. We conclude that the receptors use two pathways, one leading directly to the lytic vacuole and the other going via the plasma membrane, and that the Ileu-608 Met-609 motif has a role in the retrieval step in both pathways.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.