ArticleThe Journal of biological chemistry2010
The carboxyl-terminal end of Cox1 is required for feedback assembly regulation of Cox1 synthesis in Saccharomyces cerevisiae mitochondria.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 39 citations in OpenAlex.
- Structure determination and dual targeting of a plant TACO1 identifies its ancient role as an organelle translation regulator.bioRxiv : the preprint server for biology · 2026Article
- The cytochromeLife science alliance · 2023Article
- DPC29 promotes post-initiation mitochondrial translation in Saccharomyces cerevisiae.Nucleic acids research · 2023Article
- The yeast protein Mam33 functions in the assembly of the mitochondrial ribosome.The Journal of biological chemistry · 2019Article
- Cardiomyopathy-associated mutation in the ADP/ATP carrier reveals translation-dependent regulation of cytochrome c oxidase activity.Molecular biology of the cell · 2018Article
- Cbp3 and Cbp6 are dispensable for synthesis regulation of cytochromeThe Journal of biological chemistry · 2018Article
- MrpL35, a mitospecific component of mitoribosomes, plays a key role in cytochromeMolecular biology of the cell · 2017Article
- The Cox1 C-terminal domain is a central regulator of cytochromeThe Journal of biological chemistry · 2017Article
- A Novel Function of Pet54 in Regulation of Cox1 Synthesis in Saccharomyces cerevisiae Mitochondria.The Journal of biological chemistry · 2016Article
- Mitochondrial Cytochrome c Oxidase Biogenesis Is Regulated by the Redox State of a Heme-Binding Translational Activator.Antioxidants & redox signaling · 2016Article
- Mam33 promotes cytochrome c oxidase subunit I translation in Saccharomyces cerevisiae mitochondria.Molecular biology of the cell · 2015Article
- Redox and reactive oxygen species regulation of mitochondrial cytochrome C oxidase biogenesis.Antioxidants & redox signaling · 2013Review
- The heme a synthase Cox15 associates with cytochrome c oxidase assembly intermediates during Cox1 maturation.Molecular and cellular biology · 2013Article
- Article
- A heme-sensing mechanism in the translational regulation of mitochondrial cytochrome c oxidase biogenesis.Cell metabolism · 2012Article
- Mitochondrial protein synthesis, import, and assembly.Genetics · 2012Article
- Oligomerization of heme o synthase in cytochrome oxidase biogenesis is mediated by cytochrome oxidase assembly factor Coa2.The Journal of biological chemistry · 2012Article
- Biogenesis and assembly of eukaryotic cytochrome c oxidase catalytic core.Biochimica et biophysica acta · 2012Review
- Schizosaccharomyces pombe homologs of the Saccharomyces cerevisiae mitochondrial proteins Cbp6 and Mss51 function at a post-translational step of respiratory complex biogenesis.Mitochondrion · 2012Article
- Nonsense mutations in the COX1 subunit impair the stability of respiratory chain complexes rather than their assembly.The EMBO journal · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Synthesis of the largest cytochrome c oxidase (CcO) subunit, Cox1, on yeast mitochondrial ribosomes is coupled to assembly of CcO. The translational activator Mss51 is sequestered in early assembly intermediate complexes by an interaction with Cox14 that depends on the presence of newly synthesized Cox1. If CcO assembly is prevented, the level of Mss51 available for translational activation is reduced. We deleted the C-terminal 11 or 15 residues of Cox1 by site-directed mutagenesis of mtDNA. Although these deletions did not prevent respiratory growth of yeast, they eliminated the assembly-feedback control of Cox1 synthesis. Furthermore, these deletions reduced the strength of the Mss51-Cox14 interaction as detected by co-immunoprecipitation, confirming the importance of the Cox1 C-terminal residues for Mss51 sequestration. We surveyed a panel of mutations that block CcO assembly for the strength of their effect on Cox1 synthesis, both by pulse labeling and expression of the ARG8(m) reporter fused to COX1. Deletion of the nuclear gene encoding Cox6, one of the first subunits to be added to assembling CcO, caused the most severe reduction in Cox1 synthesis. Deletion of the C-terminal 15 amino acids of Cox1 increased Cox1 synthesis in the presence of each of these mutations, except pet54. Our data suggest a novel activity of Pet54 required for normal synthesis of Cox1 that is independent of the Cox1 C-terminal end.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.