ArticleJournal of neuroscience research2010
Localized alteration of microtubule polymerization in response to guidance cues.
Article in Journal of neuroscience research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 22 citations in OpenAlex.
- Alpha 7 nicotinic receptors attenuate neurite development through calcium activation of calpain at the growth cone.PloS one · 2018Article
- CSPGs inhibit axon branching by impairing mitochondria-dependent regulation of actin dynamics and axonal translation.Developmental neurobiology · 2017Article
- Mycalolide B dissociates dynactin and abolishes retrograde axonal transport of dense-core vesicles.Molecular biology of the cell · 2015Article
- Microtubule dynamics at the growth cone are mediated by α7 nicotinic receptor activation of a Gαq and IP3 receptor pathway.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2014Article
- Reelin promotes microtubule dynamics in processes of developing neurons.Histochemistry and cell biology · 2013Article
- Global analysis of neuronal phosphoproteome regulation by chondroitin sulfate proteoglycans.PloS one · 2013Article
- Corneal sulfated glycosaminoglycans and their effects on trigeminal nerve growth cone behavior in vitro: roles for ECM in cornea innervation.Investigative ophthalmology & visual science · 2012Article
- Myosin II activity regulates neurite outgrowth and guidance in response to chondroitin sulfate proteoglycans.Journal of neurochemistry · 2012Article
- plusTipTracker: Quantitative image analysis software for the measurement of microtubule dynamics.Journal of structural biology · 2011Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Inhibition of microtubule dynamic instability prevents growth cone turning in response to guidance cues, yet specific changes in microtubule polymerization as growth cones encounter boundaries have not been investigated. In this study, we examined the rate and direction of microtubule polymerization in response to soluble nerve growth factor (NGF) and immobilized chondroitin sulfate proteoglycans (CSPGs) by expressing enhanced GFP-EB3 in rat pheochromocytoma (PC12) cells. GFP-EB3 comets were monitored in live cells using time-lapse epifluorescent microscopy. With an automated tracking system, the rate of microtubule polymerization was calculated as the frame-to-frame displacement of EB3 comets. Our results demonstrate that the rate of microtubule polymerization is increased following NGF treatment, whereas contact with CSPGs decreases microtubule polymerization rates. This reduction in microtubule polymerization rates was specifically localized to neurites in direct contact with CSPGs and not at noncontacting neurites. Additionally, we found an increase in the percentage of microtubules polymerizing in the retrograde direction in neurites at CSPG boundaries, with a concomitant decrease in the rate of retrograde microtubule polymerization. These results implicate localized changes in microtubule dynamics as an important component of the growth cone response to guidance cues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.