ArticleJournal of orthopaedic research : official publication of the Orthopaedic Research Society2011
Titanium particles activate toll-like receptor 4 independently of lipid rafts in RAW264.7 murine macrophages.
Article in Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.
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Who cites it
10 citing papers in PubMed, 1 synthesis or guideline pooled it, 23 citations in OpenAlex.
- Are clinical findings of systemic titanium dispersion following implantation explained by available in vitro evidence? An evidence-based analysis.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2017Pooled it
- Lipopolysaccharide uptake is augmented in lipopolysaccharide-tolerant mouse macrophage-like cells via increased CD14 expression.FEBS open bio · 2026Article
- Titania nanospikes activate macrophage phagocytosis by ligand-independent contact stimulation.Scientific reports · 2022Article
- Ma Xing Shi Gan Decoction Attenuates PM2.5 Induced Lung Injury via Inhibiting HMGB1/TLR4/NFκB Signal Pathway in Rat.Frontiers in pharmacology · 2019Article
- SPHK-2 Promotes the Particle-Induced Inflammation of RAW264.7 by Maintaining Consistent Expression of TNF-α and IL-6.Inflammation · 2018Article
- Native low density lipoprotein promotes lipid raft formation in macrophages.Molecular medicine reports · 2016Article
- Polyethylene wear particles induce TLR 2 upregulation in the synovial layer of mice.Journal of materials science. Materials in medicine · 2014Article
- Macrophages-Key cells in the response to wear debris from joint replacements.Journal of biomedical materials research. Part A · 2013Review
- Micrometer-sized titanium particles can induce potent Th2-type responses through TLR4-independent pathways.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- Mediators of the inflammatory response to joint replacement devices.Nature reviews. Rheumatology · 2011Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Adherent pathogen-associated molecular patterns (PAMPs) act through toll-like receptor 2 (TLR2) and TLR4 to increase the biological activity of orthopedic wear particles in cell culture and animal models of implant loosening. This study tested whether this is dependent on TLR association with lipid rafts as reported for the response to soluble TLR ligands. For this purpose, RAW264.7 murine macrophages were activated by exposure to titanium particles with adherent PAMPs, soluble lipopolysaccharide (LPS), soluble lipotecichoic acid (LTA), or heat-killed bacteria that had been extensively washed to remove soluble PAMPs. Lipid rafts were isolated by two independent methods and the location of TLR4 and TLR2 was analyzed by Western blotting. The cognate TLRs associated with lipid rafts when the macrophages were activated with soluble LPS and LTA but not after stimulation with either titanium particles with adherent PAMPs or heat-killed bacteria. The lipid raft disruptor, methyl-β-cyclodextrin, dose-dependently inhibited TNF-α release in response to LPS but had no affect on TNF-α release in response to titanium particles with adherent PAMPs. We conclude, therefore, that titanium particles with adherent PAMPs and heat-killed bacteria activate TLR2 and TLR4 in macrophages without inducing either TLR to associate with lipid rafts. These results have important implications for the mechanisms of orthopedic implant loosening as well the mechanisms for TLR activation in other inflammatory situations.
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Registered trials
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