ArticleMolecular vision2010
Effect of down-regulation of aquaporins in human corneal endothelial and epithelial cell lines.
Article in Molecular vision, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
- Exploring corneal endothelial cell dynamics: structure, functions and therapeutic approaches.Molecular biology reports · 2025Review
- Co-differentiation and enrichment of corneal endothelial cells and keratocytes from human pluripotent stem cells.Scientific reports · 2025Article
- Aquaporins in the Cornea.International journal of molecular sciences · 2024Review
- Role of aquaporins in corneal healing post chemical injury.Experimental eye research · 2023Review
- Aquaporin 5 promotes corneal wound healing.Experimental eye research · 2018Article
- Aquaporin 1-mediated changes in pulmonary arterial smooth muscle cell migration and proliferation involve β-catenin.American journal of physiology. Lung cellular and molecular physiology · 2017Article
- In vitro biomimetic platforms featuring a perfusion system and 3D spheroid culture promote the construction of tissue-engineered corneal endothelial layers.Scientific reports · 2017Article
- Aquaporin-5: from structure to function and dysfunction in cancer.Cellular and molecular life sciences : CMLS · 2016Review
- Effect of dobutamine on lung aquaporin 5 in endotoxine shock-induced acute lung injury rabbit.Journal of thoracic disease · 2015Article
- Potential Reparative Role of Resident Adult Renal Stem/Progenitor Cells in Acute Kidney Injury.BioResearch open access · 2015Review
- Aquaporins in the eye: expression, function, and roles in ocular disease.Biochimica et biophysica acta · 2014Review
- The expression and role of Aquaporin 5 in esophageal squamous cell carcinoma.Journal of gastroenterology · 2014Article
- Genome-wide host responses against infectious laryngotracheitis virus vaccine infection in chicken embryo lung cells.BMC genomics · 2012Article
- 3-Bromopyruvate antagonizes effects of lactate and pyruvate, synergizes with citrate and exerts novel anti-glioma effects.Journal of bioenergetics and biomembranes · 2012Article
- Article
- Spatial expression of aquaporin 5 in mammalian cornea and lens, and regulation of its localization by phosphokinase A.Molecular vision · 2012Article
- Aquaporin-1 promotes angiogenesis, fibrosis, and portal hypertension through mechanisms dependent on osmotically sensitive microRNAs.The American journal of pathology · 2011Article
- Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe purpose of this study was to determine the effects of down-regulation of Aquaporin 1 (AQP1) and Aquaporin 5 (AQP5) on cell proliferation and migration in human corneal endothelial (HCEC) and human corneal epithelial (CEPI17) cell lines, respectively.
methodsAQP1 and AQP5 were down regulated using siRNA following lipofectamine-mediated transfection in corneal endothelial and epithelial cells, respectively. Down-regulation was confirmed using RT-PCR, indirect immunofluorescence, and immunoblot analysis. Total internal reflection fluorescence (TIRF) microscopy was used to detect cell surface aquaporin expression. Cell proliferation was determined by SRB (sulfrodamine B) assay. Cell migration was determined by in vitro wound healing and migration assay.
resultsIn HCEC cells, AQP1 was localized to the cytosol as well as cell membrane and its down-regulation resulted in decreased cell proliferation and migration with a significant decrease in phosphorylated ERK (pERK). In CEPI17 cells AQP5 protein expression was also localized to cytosol as well as cell membrane. AQP5 down-regulation resulted in an increase in proliferation and cell migration with no significant difference in pERK.
conclusionsAQP1 plays a role in HCEC proliferation and migration via the ERK signaling pathway and therefore may have significant implications in corneal endothelial dysfunction whereas; AQP5 may play an indirect role in human corneal epithelial cell proliferation and migration.
Indexed as
Identifiers
20806077PMC2925904W2164887884What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.