ArticleACS chemical neuroscience2010
Mapping neuropeptide expression by mass spectrometry in single dissected identified neurons from the dorsal ganglion of the nematode Ascaris suum.
Article in ACS chemical neuroscience, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it.
- MALDI-TOF mass spectrometry as a diagnostic tool in human and veterinary helminthology: a systematic review.Parasites & vectors · 2019Pooled it
- Single-tissue proteomics inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Behavioral States.Genetics · 2020Review
- Different Bioactive Neuropeptides are Expressed in Two Sub-Classes of GABAergic RME Nerve Ring Motorneurons in Ascaris suum.ACS chemical neuroscience · 2018Article
- Neuroendocrine modulation sustains theeLife · 2016Article
- Unraveling flp-11/flp-32 dichotomy in nematodes.International journal for parasitology · 2016Article
- C. elegans Stress-Induced Sleep Emerges from the Collective Action of Multiple Neuropeptides.Current biology : CB · 2016Article
- Single Cell Peptide Heterogeneity of Rat Islets of Langerhans.ACS chemical biology · 2016Article
- Mass Spectrometry of Single GABAergic Somatic Motorneurons Identifies a Novel Inhibitory Peptide, As-NLP-22, in the Nematode Ascaris suum.Journal of the American Society for Mass Spectrometry · 2015Article
- Mass spectrometry-based characterization of endogenous peptides and metabolites in small volume samples.Biochimica et biophysica acta · 2015Review
- Different neuropeptides are expressed in different functional subsets of cholinergic excitatory motorneurons in the nematode Ascaris suum.ACS chemical neuroscience · 2015Article
- FMRFamide-like FLP-13 neuropeptides promote quiescence following heat stress in Caenorhabditis elegans.Current biology : CB · 2014Article
- The FMRFamide-Like Peptide Family in Nematodes.Frontiers in endocrinology · 2014Review
- Small-volume analysis of cell-cell signaling molecules in the brain.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2014Review
- Three independent techniques localize expression of transcript afp-11 and its bioactive peptide products to the paired AVK neurons in Ascaris suum: in situ hybridization, immunocytochemistry, and single cell mass spectrometry.ACS chemical neuroscience · 2013Article
- Comparison of NIMS and MALDI platforms for neuropeptide and lipid mass spectrometric imaging inAnalytical methods : advancing methods and applications · 2013Article
- Parasite neuropeptide biology: Seeding rational drug target selection?International journal for parasitology. Drugs and drug resistance · 2012Review
- Probing neuropeptide signaling at the organ and cellular domains via imaging mass spectrometry.Journal of proteomics · 2012Review
- Changes in cyclic nucleotides, locomotory behavior, and body length produced by novel endogenous neuropeptides in the parasitic nematode Ascaris suum.Molecular and biochemical parasitology · 2011Article
- Recent advances in single-cell MALDI mass spectrometry imaging and potential clinical impact.Expert review of proteomics · 2011Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
We have developed a method for dissecting single neurons from the nematode Ascaris suum, in order to determine their peptide content by mass spectrometry (MS). In this paper, we use MALDI-TOF MS and tandem MS to enumerate and sequence the peptides present in the two neurons, ALA and RID, that comprise the dorsal ganglion. We compare the peptide content determined by MS with the results of immunocytochemistry and in situ hybridization of previously isolated peptides AF2, AF8 and 6 peptides encoded by the afp-1 transcript. We find complete agreement between the three techniques, which validates single neuron MS as a method for peptide localization. We also discovered and sequenced 6 novel peptides in the ALA neuron. Cloning of cDNAs and database searching of Genomic Survey Sequences showed that transcript afp-12 encodes peptide AF36 (VPSAADMMIRFamide), and afp-13 encodes AF19 (AEGLSSPLIRFamide), AF34 (DSKLMDPLIRFamide), AF35 (DPQQRIVTDETVLRFamide), and 3 non-amidated peptides (PepTT, PepTL, and PepGE). We have found no similarities with reported peptide expression in the nematode Caenorhabditis elegans. This method promises to be ideally suited for determining the peptide content of each of the 298 neurons in the nervous system of this nematode.
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Registered trials
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