ArticleBiomicrofluidics2010
A dielectrophoretic chip with a roughened metal surface for on-chip surface-enhanced Raman scattering analysis of bacteria.
Article in Biomicrofluidics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Recent advances in surface enhanced Raman spectroscopy for bacterial pathogen identifications.Journal of advanced research · 2023Pooled it
- Review
- Rapid Lipid Content Screening inMicromachines · 2021Article
- Combined negative dielectrophoresis with a flexible SERS platform as a novel strategy for rapid detection and identification of bacteria.Analytical and bioanalytical chemistry · 2021Article
- Point-of-care microfluidic devices for pathogen detection.Biosensors & bioelectronics · 2018Review
- Surface enhanced Raman scattering (SERS) based biomicrofluidics systems for trace protein analysis.Biomicrofluidics · 2018Review
- Review: Microbial analysis in dielectrophoretic microfluidic systems.Analytica chimica acta · 2017Review
- Alternative cDEP Design to Facilitate Cell Isolation for Identification by Raman Spectroscopy.Sensors (Basel, Switzerland) · 2017Article
- Detection of extremely low concentration waterborne pathogen using a multiplexing self-referencing SERS microfluidic biosensor.Journal of biological engineering · 2017Article
- Rapid separation of bacteria from blood-review and outlook.Biotechnology progress · 2016Review
- Accelerated detection of viral particles by combining AC electric field effects and micro-Raman spectroscopy.Sensors (Basel, Switzerland) · 2015Article
- Development of flow through dielectrophoresis microfluidic chips for biofuel production: Sorting and detection of microalgae with different lipid contents.Biomicrofluidics · 2014Article
- Electrokinetic trapping and surface enhanced Raman scattering detection of biomolecules using optofluidic device integrated with a microneedles array.Biomicrofluidics · 2013Article
- Rapid (<5 min) identification of pathogen in human blood by electrokinetic concentration and surface-enhanced Raman spectroscopy.Scientific reports · 2013Article
- A practical guide for the fabrication of microfluidic devices using glass and silicon.Biomicrofluidics · 2012Article
- Study on the use of dielectrophoresis and electrothermal forces to produce on-chip micromixers and microconcentrators.Biomicrofluidics · 2012Article
- Three-dimensional cellular focusing utilizing a combination of insulator-based and metallic dielectrophoresis.Biomicrofluidics · 2011Article
- An insulator-based dielectrophoretic microdevice for the simultaneous filtration and focusing of biological cells.Biomicrofluidics · 2011Article
- Antibiotic susceptibility test based on the dielectrophoretic behavior of elongated Escherichia coli with cephalexin treatment.Biomicrofluidics · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present an analysis of the results of in situ surface-enhanced Raman scattering (SERS) of bacteria using a microfluidic chip capable of continuously sorting and concentrating bacteria via three-dimensional dielectrophoresis (DEP). Microchannels were made by sandwiching DEP microelectrodes between two glass slides. Avoiding the use of a metal nanoparticle suspension, a roughened metal surface is integrated into the DEP-based microfluidic chip for on-chip SERS detection of bacteria. On the upper surface of the slide, a roughened metal shelter was settled in front of the DEP concentrator to enhance Raman scattering. Similarly, an electrode-patterned bottom layer fabricated on a thin cover-slip was used to reduce fluorescence noise from the glass substrate. Gram positive (Staphylococcus aureus) and Gram negative (Pseudomonas aeruginosa) bacteria were effectively distinguished in the SERS spectral data. Staphylococcus aureus (concentration of 10(6) CFUml) was continuously separated and concentrated via DEP out of a sample of blood cells. At a flow rate of 1 mulmin, the bacteria were highly concentrated at the roughened surface and ready for on-chip SERS analysis within 3 min. The SERS data were successfully amplified by one order of magnitude and analyzed within a few minutes, resulting in the detection of signature peaks of the respective bacteria.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.