ArticlePloS one2010
Autonomous bioluminescent expression of the bacterial luciferase gene cassette (lux) in a mammalian cell line.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed, 155 citations in OpenAlex.
- Labeling and imaging of neurons with a genetically encoded autonomous bioluminescence system.Scientific reports · 2026Article
- Bacterial bioluminescence for the real time andAntimicrobial agents and chemotherapy · 2026Article
- Luminescence-Based Strategies for Detecting β-Lactamase Activity: A Review of the Last Decade.Life (Basel, Switzerland) · 2026Review
- Protein engineering: status report.Protein engineering, design & selection : PEDS · 2026Review
- Optical and Photoacoustic Imaging.Recent results in cancer research. Fortschritte der Krebsforschung. Progres dans les recherches sur le cancer · 2026Review
- Autonomous Bioluminescence Systems: From Molecular Mechanisms to Emerging Applications.JACS Au · 2025Review
- Autonomous bioluminescence emission from transgenic mice.Science advances · 2025Article
- Autonomous multicolor bioluminescence imaging in bacteria, mammalian, and plant hosts.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- A Comprehensive Exploration of Bioluminescence Systems, Mechanisms, and Advanced Assays for Versatile Applications.Biochemistry research international · 2024Review
- Using bioluminescence to image gene expression and spontaneous behavior in freely moving mice.PloS one · 2023Article
- Generation of bright autobioluminescent bacteria by chromosomal integration of the improved lux operon ilux2.Scientific reports · 2022Article
- An Autonomous Molecular Bioluminescent Reporter (AMBER) for Voltage Imaging in Freely Moving Animals.Advanced biology · 2021Article
- Enhanced brightness of bacterial luciferase by bioluminescence resonance energy transfer.Scientific reports · 2021Article
- Improvements in Smartphone and Night Vision Imaging Technologies Enable Low Cost, On-Site Assays of Bioluminescent Cells.Frontiers in bioengineering and biotechnology · 2021Article
- A Photocleavable Contrast Agent for Light-Responsive MRI.Pharmaceuticals (Basel, Switzerland) · 2020Article
- Real-time tracking of stem cell viability, proliferation, and differentiation with autonomous bioluminescence imaging.BMC biology · 2020Article
- Autonomous bioluminescence imaging of single mammalian cells with the bacterial bioluminescence system.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Synthetic metabolic computation in a bioluminescence-sensing system.Nucleic acids research · 2019Article
- Ultrasound imaging of gene expression in mammalian cells.Science (New York, N.Y.) · 2019Article
- Development and Applications of Bioluminescent and Chemiluminescent Reporters and Biosensors.Annual review of analytical chemistry (Palo Alto, Calif.) · 2019Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundThe bacterial luciferase (lux) gene cassette consists of five genes (luxCDABE) whose protein products synergistically generate bioluminescent light signals exclusive of supplementary substrate additions or exogenous manipulations. Historically expressible only in prokaryotes, the lux operon was re-synthesized through a process of multi-bicistronic, codon-optimization to demonstrate for the first time self-directed bioluminescence emission in a mammalian HEK293 cell line in vitro and in vivo. METHODOLOGY/PRINCIPAL
findingsAutonomous in vitro light production was shown to be 12-fold greater than the observable background associated with untransfected control cells. The availability of reduced riboflavin phosphate (FMNH(2)) was identified as the limiting bioluminescence substrate in the mammalian cell environment even after the addition of a constitutively expressed flavin reductase gene (frp) from Vibrio harveyi. FMNH(2) supplementation led to a 151-fold increase in bioluminescence in cells expressing mammalian codon-optimized luxCDE and frp genes. When injected subcutaneously into nude mice, in vivo optical imaging permitted near instantaneous light detection that persisted independently for the 60 min length of the assay with negligible background. CONCLUSIONS/SIGNIFICANCE: The speed, longevity, and self-sufficiency of lux expression in the mammalian cellular environment provides a viable and powerful alternative for real-time target visualization not currently offered by existing bioluminescent and fluorescent imaging technologies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.